1 Ocak 2018 Dergi makalesi Açık Erişim

Vardar, Deniz Ozkan; Aydin, Sevtap; Hocaoglu, Ibrahim; Acar, Funda Havva Yagci; Basaran, Nursen

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  <dc:creator>Vardar, Deniz Ozkan</dc:creator>
  <dc:creator>Aydin, Sevtap</dc:creator>
  <dc:creator>Hocaoglu, Ibrahim</dc:creator>
  <dc:creator>Acar, Funda Havva Yagci</dc:creator>
  <dc:creator>Basaran, Nursen</dc:creator>
  <dc:date>2018-01-01</dc:date>
  <dc:description>Quantum dots (QDs) are highly promising nanomaterials in bioimaging system because of their bright fluorescence, broad UV excitation, narrow emission band, and high photostability. Recently, there is a great activity on Ag2S quantum dots for both imaging and drug/gene delivery due to the potential of having a better cytocompatability and near infrared luminescence. 2-Mercaptopropionic acid (2 MP A)-coated silver sulfide (Ag2S) QDs were reported as the most luminescent, stable, anionic Ag2S QDs in the literature. In this study, we aim to determine the cytotoxicity of 2 MP A/Ag2S in Chinese hamster lung fibroblast (V79) cells. The genotoxic and apoptotic effects of 2 MP A/Ag2S QDs were assessed by the alkaline single cell electrophoresis assay and real time polymerase chain reaction techniques, respectively. The cell viability decreased above 200 mu g/ml and 800 mu g/ml for MTT tetrazolium and neural red uptake assays, respectively. DNA damage was not observed by 2 MP A/Ag2S QDs at the studied concentration levels (5-2000 mu g/ml). The levels of mRNA expression of p53, caspase 3, caspase 9, box, bcl-2, survivin were not changed by 2 MP A/Ag2S QDs below IC50 (around 1000 mu g/ml). Hence, 2 MP A/Ag2S QDs did not show any cytotoxic or genotoxic effects in V79 cells at lower doses. We conclude that the biocompatibility of 2 MP A/Ag2S ODs makes them suitable for cell labeling applications.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/89793</dc:identifier>
  <dc:identifier>oai:zenodo.org:89793</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>CHEMICO-BIOLOGICAL INTERACTIONS 291 212-219</dc:source>
  <dc:title>Effects of silver sulfide quantum dots coated with 2-mercaptopropionic acid on genotoxic and apoptotic pathways in vitro</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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