1 Ocak 2018 Dergi makalesi Açık Erişim

Vardar, Deniz Ozkan; Aydin, Sevtap; Hocaoglu, Ibrahim; Acar, Funda Havva Yagci; Basaran, Nursen

Citation Style Language JSON

{
  "DOI": "10.1016/j.cbi.2018.06.032", 
  "abstract": "Quantum dots (QDs) are highly promising nanomaterials in bioimaging system because of their bright fluorescence, broad UV excitation, narrow emission band, and high photostability. Recently, there is a great activity on Ag2S quantum dots for both imaging and drug/gene delivery due to the potential of having a better cytocompatability and near infrared luminescence. 2-Mercaptopropionic acid (2 MP A)-coated silver sulfide (Ag2S) QDs were reported as the most luminescent, stable, anionic Ag2S QDs in the literature. In this study, we aim to determine the cytotoxicity of 2 MP A/Ag2S in Chinese hamster lung fibroblast (V79) cells. The genotoxic and apoptotic effects of 2 MP A/Ag2S QDs were assessed by the alkaline single cell electrophoresis assay and real time polymerase chain reaction techniques, respectively. The cell viability decreased above 200 mu g/ml and 800 mu g/ml for MTT tetrazolium and neural red uptake assays, respectively. DNA damage was not observed by 2 MP A/Ag2S QDs at the studied concentration levels (5-2000 mu g/ml). The levels of mRNA expression of p53, caspase 3, caspase 9, box, bcl-2, survivin were not changed by 2 MP A/Ag2S QDs below IC50 (around 1000 mu g/ml). Hence, 2 MP A/Ag2S QDs did not show any cytotoxic or genotoxic effects in V79 cells at lower doses. We conclude that the biocompatibility of 2 MP A/Ag2S ODs makes them suitable for cell labeling applications.", 
  "author": [
    {
      "family": "Vardar", 
      "given": " Deniz Ozkan"
    }, 
    {
      "family": "Aydin", 
      "given": " Sevtap"
    }, 
    {
      "family": "Hocaoglu", 
      "given": " Ibrahim"
    }, 
    {
      "family": "Acar", 
      "given": " Funda Havva Yagci"
    }, 
    {
      "family": "Basaran", 
      "given": " Nursen"
    }
  ], 
  "container_title": "CHEMICO-BIOLOGICAL INTERACTIONS", 
  "id": "89793", 
  "issued": {
    "date-parts": [
      [
        2018, 
        1, 
        1
      ]
    ]
  }, 
  "page": "212-219", 
  "title": "Effects of silver sulfide quantum dots coated with 2-mercaptopropionic acid on genotoxic and apoptotic pathways in vitro", 
  "type": "article-journal", 
  "volume": "291"
}