1 Ocak 2015 Dergi makalesi Açık Erişim

Kip, Cigdem; Erkakan, Damla; Gokaltun, Aslihan; Celebi, Bekir; Tuncel, Ali

Dublin Core

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  <dc:creator>Kip, Cigdem</dc:creator>
  <dc:creator>Erkakan, Damla</dc:creator>
  <dc:creator>Gokaltun, Aslihan</dc:creator>
  <dc:creator>Celebi, Bekir</dc:creator>
  <dc:creator>Tuncel, Ali</dc:creator>
  <dc:date>2015-01-01</dc:date>
  <dc:description>Poly(3-chloro-2-hydroxypropyl methacrylate-co-ethylene dimethacrylate), poly(HPMA-Cl-co-EDMA) capillary monolith was proposed as a reactive starting material with tailoring flexibility for the preparation of monolithic stationary phases. The reactive capillary monolith was synthesized by free radical copolymerization of 3-chloro-2-hydroxypropyl methacrylate (HPMA-Cl) and ethylene dimethacrylate (EDMA). The mean pore size, the specific surface area and the permeability of poly(HPMA-Cl-co-EDMA) monoliths were controlled by adjusting porogen/monomer volume ratio, porogen composition and polymerization temperature. The porogen/monomer volume ratio was found as the most effective factor controlling the porous properties of poly(HPMA-Cl-co-EDMA) monolith. Triethanolamine (TEA-OH) functionalized polymethacrylate monoliths were prepared by using the reactive chloropropyl group of poly(HPMA-Cl-co-EDMA) monolith via one-pot and simple post-functionalization process. Poly(HPMA-Cl-co-EDMA) monolith reacted with TEA-OH was evaluated as a stationary phase in nano-hydrophilic interaction chromatography (nano-HILIC). Nucleotides, nucleosides and benzoic acid derivatives were satisfactorily separated with the plate heights up to 20 mu m. TEA-OH attached-poly(HPMA-Cl-co-EDIVIA) monolith showed a reproducible and stable retention behaviour in nano-HILIC runs. However, a decrease in the column performance (i.e. an increase in the plate height) was observed with the increasing retention factor. Hence "retention-dependent column efficiency" behaviour was shown for HILIC mode using the chromatographic data collected with the polymer based monolith synthesized. (C) 2015 Elsevier B.V. All rights reserved.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/82947</dc:identifier>
  <dc:identifier>oai:zenodo.org:82947</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>JOURNAL OF CHROMATOGRAPHY A 1396 86-97</dc:source>
  <dc:title>Synthesis of a reactive polymethacrylate capillary monolith and its use as a starting material for the preparation of a stationary phase for hydrophilic interaction chromatography</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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