1 Ocak 2015 Dergi makalesi Açık Erişim

Kip, Cigdem; Erkakan, Damla; Gokaltun, Aslihan; Celebi, Bekir; Tuncel, Ali

Citation Style Language JSON

{
  "DOI": "10.1016/j.chroma.2015.04.005", 
  "abstract": "Poly(3-chloro-2-hydroxypropyl methacrylate-co-ethylene dimethacrylate), poly(HPMA-Cl-co-EDMA) capillary monolith was proposed as a reactive starting material with tailoring flexibility for the preparation of monolithic stationary phases. The reactive capillary monolith was synthesized by free radical copolymerization of 3-chloro-2-hydroxypropyl methacrylate (HPMA-Cl) and ethylene dimethacrylate (EDMA). The mean pore size, the specific surface area and the permeability of poly(HPMA-Cl-co-EDMA) monoliths were controlled by adjusting porogen/monomer volume ratio, porogen composition and polymerization temperature. The porogen/monomer volume ratio was found as the most effective factor controlling the porous properties of poly(HPMA-Cl-co-EDMA) monolith. Triethanolamine (TEA-OH) functionalized polymethacrylate monoliths were prepared by using the reactive chloropropyl group of poly(HPMA-Cl-co-EDMA) monolith via one-pot and simple post-functionalization process. Poly(HPMA-Cl-co-EDMA) monolith reacted with TEA-OH was evaluated as a stationary phase in nano-hydrophilic interaction chromatography (nano-HILIC). Nucleotides, nucleosides and benzoic acid derivatives were satisfactorily separated with the plate heights up to 20 mu m. TEA-OH attached-poly(HPMA-Cl-co-EDIVIA) monolith showed a reproducible and stable retention behaviour in nano-HILIC runs. However, a decrease in the column performance (i.e. an increase in the plate height) was observed with the increasing retention factor. Hence \"retention-dependent column efficiency\" behaviour was shown for HILIC mode using the chromatographic data collected with the polymer based monolith synthesized. (C) 2015 Elsevier B.V. All rights reserved.", 
  "author": [
    {
      "family": "Kip", 
      "given": " Cigdem"
    }, 
    {
      "family": "Erkakan", 
      "given": " Damla"
    }, 
    {
      "family": "Gokaltun", 
      "given": " Aslihan"
    }, 
    {
      "family": "Celebi", 
      "given": " Bekir"
    }, 
    {
      "family": "Tuncel", 
      "given": " Ali"
    }
  ], 
  "container_title": "JOURNAL OF CHROMATOGRAPHY A", 
  "id": "82947", 
  "issued": {
    "date-parts": [
      [
        2015, 
        1, 
        1
      ]
    ]
  }, 
  "page": "86-97", 
  "title": "Synthesis of a reactive polymethacrylate capillary monolith and its use as a starting material for the preparation of a stationary phase for hydrophilic interaction chromatography", 
  "type": "article-journal", 
  "volume": "1396"
}