1 Ocak 2015 Dergi makalesi Açık Erişim

Lenkey, Nora; Kirizs, Tekla; Holderith, Noemi; Mate, Zoltan; Szabo, Gabor; Vizi, E. Sylvester; Hajos, Norbert; Nusser, Zoltan

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{
  "DOI": "10.1038/ncomms7557", 
  "abstract": "The release of GABA from cholecystokinin-containing interneurons is modulated by type-1 cannabinoid receptors (CB1). Here we tested the hypothesis that the strength of CB1-mediated modulation of GABA release is related to the CB1 content of axon terminals. Basket cell boutons have on average 78% higher CB1 content than those of dendritic-layer-innervating (DLI) cells, a consequence of larger bouton surface and higher CB1 density. The CB1 antagonist AM251 caused a 54% increase in action potential-evoked [Ca2+] in boutons of basket cells, but not in DLI cells. However, the effect of AM251 did not correlate with CB1 immunoreactivity of individual boutons. Moreover, a CB1 agonist decreased [Ca2+] in a cell type-and CB1-content-independent manner. Replica immunogold labelling demonstrated the colocalization of CB1 with the Cav2.2 Ca2+ channel subunit. Our data suggest that only a subpopulation of CB(1)s, within nanometre distances from their target Cav2.2 channels, are responsible for endocannabinoid-mediated modulation of GABA release.", 
  "author": [
    {
      "family": "Lenkey", 
      "given": " Nora"
    }, 
    {
      "family": "Kirizs", 
      "given": " Tekla"
    }, 
    {
      "family": "Holderith", 
      "given": " Noemi"
    }, 
    {
      "family": "Mate", 
      "given": " Zoltan"
    }, 
    {
      "family": "Szabo", 
      "given": " Gabor"
    }, 
    {
      "family": "Vizi", 
      "given": " E. Sylvester"
    }, 
    {
      "family": "Hajos", 
      "given": " Norbert"
    }, 
    {
      "family": "Nusser", 
      "given": " Zoltan"
    }
  ], 
  "container_title": "NATURE COMMUNICATIONS", 
  "id": "81947", 
  "issued": {
    "date-parts": [
      [
        2015, 
        1, 
        1
      ]
    ]
  }, 
  "title": "Tonic endocannabinoid-mediated modulation of GABA release is independent of the CB1 content of axon terminals", 
  "type": "article-journal", 
  "volume": "6"
}