Dergi makalesi Açık Erişim

DETERMINATION OF SERTRALINE AND ITS METABOLITE BY HIGH-PRESSURE LIQUID CHROMATOGRAPHY IN PLASMA

2015    Yuce-Artun, Nazan; Ozel Kizil, Erguvan Tugba; Baskak, Bora; Ozguven, Halise Devrimci; Duydu, Yalcin; Suzen, Halit Sinan

A fast, simple and sensitive high-pressure liquid chromatography (HPLC) method with UV detection was developed for frequently prescribed antidepressant, sertraline (SERT) and its main metabolite N-desmethylsertraline (DSERT), in human plasma. SERT and DSERT were extracted by an optimized solid phase chromatographic (SPE) method using C-18 cartridges and Clomipramine was used as external standard (ES). The analytes were separated on C18, 4.6 mm x 150 mm, 5 pm column at 50 degrees C with a mobile phase of 45% acetonitrile + 55% NaH2PO4 at a flow rate of 0.4 mL/min. Detector responses monitored at 4 different wave-lengths; 200-205-210-215 nm. The method proved to be rapid and effective for the plasma sample analyses of therapeutic drug monitoring for sertraline treated patients.