1 Ocak 2019 Dergi makalesi Açık Erişim

Turan, Eylem; Sahin, Ferat; Suludere, Zekiye; Tumturk, Hayrettin

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  <dc:creator>Turan, Eylem</dc:creator>
  <dc:creator>Sahin, Ferat</dc:creator>
  <dc:creator>Suludere, Zekiye</dc:creator>
  <dc:creator>Tumturk, Hayrettin</dc:creator>
  <dc:date>2019-01-01</dc:date>
  <dc:description>This study describes the design of a novel fluoroimmunodiagnostic nanoplatform by using tannylated ferritin nanocages (TA-Fn). The tannylation process ensured the tannic acid was modified onto the ferritin nanocages without EDC, NHS or any reagents. The bare ferritin nanocages (Fn) had about 5.6 +/- 0.14 nm average diameter, while tannylated ferritin nanocages had an estimated layer thickness of about 24 +/- 2.2 nm. The FITC labelled thyroglobulin antibodies (FITC-TgAb) were conjugated to TA-Fn via multiple hydrogen bonds and hydrophobic interactions. The FITC labelled TgAb modified tannylated ferritin nanocages (FITC-TgAb-TA-Fn) had an estimated layer thickness of about 32 +/- 3.1 nm. The FITC-TgAb-TA-Fn had good selectivity and specificity to rapidly capture and detect specific antigens via fluorescence quenching with relatively low-cost engineering. The Stern-Volmer plot was used to obtain limit of detection (LOD) and limit of quantification (LOQ). The LOD was 4.3 pg mL(-1) and LOQ was 14.2 pg mL(-1) in artificial human serum medium. These values were lower than the thyroglobulin detection limit with modern assays.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/67687</dc:identifier>
  <dc:identifier>oai:zenodo.org:67687</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>SENSORS AND ACTUATORS B-CHEMICAL 300</dc:source>
  <dc:title>A fluoroimmunodiagnostic nanoplatform for thyroglobulin detection based on fluorescence quenching signal</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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