1 Ocak 2019 Dergi makalesi Açık Erişim

Turan, Eylem; Sahin, Ferat; Suludere, Zekiye; Tumturk, Hayrettin

Citation Style Language JSON

{
  "DOI": "10.1016/j.snb.2019.127052", 
  "abstract": "This study describes the design of a novel fluoroimmunodiagnostic nanoplatform by using tannylated ferritin nanocages (TA-Fn). The tannylation process ensured the tannic acid was modified onto the ferritin nanocages without EDC, NHS or any reagents. The bare ferritin nanocages (Fn) had about 5.6 +/- 0.14 nm average diameter, while tannylated ferritin nanocages had an estimated layer thickness of about 24 +/- 2.2 nm. The FITC labelled thyroglobulin antibodies (FITC-TgAb) were conjugated to TA-Fn via multiple hydrogen bonds and hydrophobic interactions. The FITC labelled TgAb modified tannylated ferritin nanocages (FITC-TgAb-TA-Fn) had an estimated layer thickness of about 32 +/- 3.1 nm. The FITC-TgAb-TA-Fn had good selectivity and specificity to rapidly capture and detect specific antigens via fluorescence quenching with relatively low-cost engineering. The Stern-Volmer plot was used to obtain limit of detection (LOD) and limit of quantification (LOQ). The LOD was 4.3 pg mL(-1) and LOQ was 14.2 pg mL(-1) in artificial human serum medium. These values were lower than the thyroglobulin detection limit with modern assays.", 
  "author": [
    {
      "family": "Turan", 
      "given": " Eylem"
    }, 
    {
      "family": "Sahin", 
      "given": " Ferat"
    }, 
    {
      "family": "Suludere", 
      "given": " Zekiye"
    }, 
    {
      "family": "Tumturk", 
      "given": " Hayrettin"
    }
  ], 
  "container_title": "SENSORS AND ACTUATORS B-CHEMICAL", 
  "id": "67687", 
  "issued": {
    "date-parts": [
      [
        2019, 
        1, 
        1
      ]
    ]
  }, 
  "title": "A fluoroimmunodiagnostic nanoplatform for thyroglobulin detection based on fluorescence quenching signal", 
  "type": "article-journal", 
  "volume": "300"
}