1 Ocak 2019 Dergi makalesi Açık Erişim

Turan, Eylem; Sahin, Ferat; Suludere, Zekiye; Tumturk, Hayrettin

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{
  "@context": "https://schema.org/", 
  "@id": 67687, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Ankara Medipol Univ, Vocat Sch Hlth Serv, Dept Med Serv & Tech, TR-06050 Ankara, Turkey", 
      "name": "Turan, Eylem"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Med & Med Devices Agcy Turkey, TR-06520 Ankara, Turkey", 
      "name": "Sahin, Ferat"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Gazi Univ, Fac Sci, Dept Biol, TR-06500 Ankara, Turkey", 
      "name": "Suludere, Zekiye"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Gazi Univ, Fac Sci, Dept Chem, TR-06500 Ankara, Turkey", 
      "name": "Tumturk, Hayrettin"
    }
  ], 
  "datePublished": "2019-01-01", 
  "description": "This study describes the design of a novel fluoroimmunodiagnostic nanoplatform by using tannylated ferritin nanocages (TA-Fn). The tannylation process ensured the tannic acid was modified onto the ferritin nanocages without EDC, NHS or any reagents. The bare ferritin nanocages (Fn) had about 5.6 +/- 0.14 nm average diameter, while tannylated ferritin nanocages had an estimated layer thickness of about 24 +/- 2.2 nm. The FITC labelled thyroglobulin antibodies (FITC-TgAb) were conjugated to TA-Fn via multiple hydrogen bonds and hydrophobic interactions. The FITC labelled TgAb modified tannylated ferritin nanocages (FITC-TgAb-TA-Fn) had an estimated layer thickness of about 32 +/- 3.1 nm. The FITC-TgAb-TA-Fn had good selectivity and specificity to rapidly capture and detect specific antigens via fluorescence quenching with relatively low-cost engineering. The Stern-Volmer plot was used to obtain limit of detection (LOD) and limit of quantification (LOQ). The LOD was 4.3 pg mL(-1) and LOQ was 14.2 pg mL(-1) in artificial human serum medium. These values were lower than the thyroglobulin detection limit with modern assays.", 
  "headline": "A fluoroimmunodiagnostic nanoplatform for thyroglobulin detection based on fluorescence quenching signal", 
  "identifier": 67687, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "A fluoroimmunodiagnostic nanoplatform for thyroglobulin detection based on fluorescence quenching signal", 
  "url": "https://aperta.ulakbim.gov.tr/record/67687"
}