1 Ocak 2019 Dergi makalesi Açık Erişim

Turan, Eylem; Sahin, Ferat; Suludere, Zekiye; Tumturk, Hayrettin

DataCite XML

<?xml version='1.0' encoding='utf-8'?>
<resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.1/metadata.xsd">
  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/67687</identifier>
  <creators>
    <creator>
      <creatorName>Turan, Eylem</creatorName>
      <givenName>Eylem</givenName>
      <familyName>Turan</familyName>
      <affiliation>Ankara Medipol Univ, Vocat Sch Hlth Serv, Dept Med Serv &amp; Tech, TR-06050 Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Sahin, Ferat</creatorName>
      <givenName>Ferat</givenName>
      <familyName>Sahin</familyName>
      <affiliation>Med &amp; Med Devices Agcy Turkey, TR-06520 Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Suludere, Zekiye</creatorName>
      <givenName>Zekiye</givenName>
      <familyName>Suludere</familyName>
      <affiliation>Gazi Univ, Fac Sci, Dept Biol, TR-06500 Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Tumturk, Hayrettin</creatorName>
      <givenName>Hayrettin</givenName>
      <familyName>Tumturk</familyName>
      <affiliation>Gazi Univ, Fac Sci, Dept Chem, TR-06500 Ankara, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>A Fluoroimmunodiagnostic Nanoplatform For Thyroglobulin Detection Based On Fluorescence Quenching Signal</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2019</publicationYear>
  <dates>
    <date dateType="Issued">2019-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/67687</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1016/j.snb.2019.127052</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">This study describes the design of a novel fluoroimmunodiagnostic nanoplatform by using tannylated ferritin nanocages (TA-Fn). The tannylation process ensured the tannic acid was modified onto the ferritin nanocages without EDC, NHS or any reagents. The bare ferritin nanocages (Fn) had about 5.6 +/- 0.14 nm average diameter, while tannylated ferritin nanocages had an estimated layer thickness of about 24 +/- 2.2 nm. The FITC labelled thyroglobulin antibodies (FITC-TgAb) were conjugated to TA-Fn via multiple hydrogen bonds and hydrophobic interactions. The FITC labelled TgAb modified tannylated ferritin nanocages (FITC-TgAb-TA-Fn) had an estimated layer thickness of about 32 +/- 3.1 nm. The FITC-TgAb-TA-Fn had good selectivity and specificity to rapidly capture and detect specific antigens via fluorescence quenching with relatively low-cost engineering. The Stern-Volmer plot was used to obtain limit of detection (LOD) and limit of quantification (LOQ). The LOD was 4.3 pg mL(-1) and LOQ was 14.2 pg mL(-1) in artificial human serum medium. These values were lower than the thyroglobulin detection limit with modern assays.</description>
  </descriptions>
</resource>