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Improved in vitro regeneration and propagation of Tunceli garlic (Allium tuncelianum L.)

Kizil, S.; Icgil, D. Y.; Khawar, K. M.


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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Kizil, S.</dc:creator>
  <dc:creator>Icgil, D. Y.</dc:creator>
  <dc:creator>Khawar, K. M.</dc:creator>
  <dc:date>2014-01-01</dc:date>
  <dc:description>Over-exploitation of endemic and threatened Tunceli garlic [Allium tuncelianum (Kollman) N. Ozhatay, B. Mathew &amp; Siraneci] for household purposes has threatened the species and requires that a reliable, improved tissue culture protocol be developed for its conservation. Leaf tips, the middle portions of leaves, leaf bases, vertically-sectioned halved or quartered bulbs, horizontally-sectioned upper and lower bulb halves, and root tip explants were cultured on 1.0x Murashige and Skoog (MS) medium containing 1.0, 2.0, 3.0, 4.0, or 5.0 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) or 1.0, 2.0, 3.0, 4.0, or 5.0 mg l(-1) 6-benzylaminopurine (BAP) plus 0.5 mg l(-1) alpha-naphthaleneacetic acid (NAA). The results indicated that root tip explants were most suitable for bulblet regeneration on 1.0x MS medium containing 5.0 mg l(-1) BAP plus 0.5 mg l(-1) NAA. All other explants failed to regenerate on different concentrations of BAP plus NAA, or on 2,4-D. The regenerated bulblets were acclimatised at 24 degrees +/- 1 degrees C and 80.0% relative humidity under growth chamber conditions, then transferred to pots containing peat-moss in a greenhouse. The results will be important for garlic breeders and researchers.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/62511</dc:identifier>
  <dc:identifier>oai:zenodo.org:62511</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>JOURNAL OF HORTICULTURAL SCIENCE &amp; BIOTECHNOLOGY 89(4) 408-414</dc:source>
  <dc:title>Improved in vitro regeneration and propagation of Tunceli garlic (Allium tuncelianum L.)</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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