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Improved in vitro regeneration and propagation of Tunceli garlic (Allium tuncelianum L.)

Kizil, S.; Icgil, D. Y.; Khawar, K. M.


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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/62511</identifier>
  <creators>
    <creator>
      <creatorName>Kizil, S.</creatorName>
      <givenName>S.</givenName>
      <familyName>Kizil</familyName>
      <affiliation>Dicle Univ, Dept Field Crops, Fac Agr, TR-21280 Diyarbakir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Icgil, D. Y.</creatorName>
      <givenName>D. Y.</givenName>
      <familyName>Icgil</familyName>
      <affiliation>Dicle Univ, Dept Field Crops, Fac Agr, TR-21280 Diyarbakir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Khawar, K. M.</creatorName>
      <givenName>K. M.</givenName>
      <familyName>Khawar</familyName>
      <affiliation>Ankara Univ, Dept Field Crops, Fac Agr, TR-06110 Ankara, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Improved In Vitro Regeneration And Propagation Of Tunceli Garlic (Allium Tuncelianum L.)</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2014</publicationYear>
  <dates>
    <date dateType="Issued">2014-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/62511</alternateIdentifier>
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    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.81043/aperta.62510</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.81043/aperta.62511</relatedIdentifier>
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  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Over-exploitation of endemic and threatened Tunceli garlic [Allium tuncelianum (Kollman) N. Ozhatay, B. Mathew &amp;amp; Siraneci] for household purposes has threatened the species and requires that a reliable, improved tissue culture protocol be developed for its conservation. Leaf tips, the middle portions of leaves, leaf bases, vertically-sectioned halved or quartered bulbs, horizontally-sectioned upper and lower bulb halves, and root tip explants were cultured on 1.0x Murashige and Skoog (MS) medium containing 1.0, 2.0, 3.0, 4.0, or 5.0 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) or 1.0, 2.0, 3.0, 4.0, or 5.0 mg l(-1) 6-benzylaminopurine (BAP) plus 0.5 mg l(-1) alpha-naphthaleneacetic acid (NAA). The results indicated that root tip explants were most suitable for bulblet regeneration on 1.0x MS medium containing 5.0 mg l(-1) BAP plus 0.5 mg l(-1) NAA. All other explants failed to regenerate on different concentrations of BAP plus NAA, or on 2,4-D. The regenerated bulblets were acclimatised at 24 degrees +/- 1 degrees C and 80.0% relative humidity under growth chamber conditions, then transferred to pots containing peat-moss in a greenhouse. The results will be important for garlic breeders and researchers.</description>
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