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Improved in vitro regeneration and propagation of Tunceli garlic (Allium tuncelianum L.)

Kizil, S.; Icgil, D. Y.; Khawar, K. M.


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{
  "URL": "https://aperta.ulakbim.gov.tr/record/62511", 
  "abstract": "Over-exploitation of endemic and threatened Tunceli garlic [Allium tuncelianum (Kollman) N. Ozhatay, B. Mathew & Siraneci] for household purposes has threatened the species and requires that a reliable, improved tissue culture protocol be developed for its conservation. Leaf tips, the middle portions of leaves, leaf bases, vertically-sectioned halved or quartered bulbs, horizontally-sectioned upper and lower bulb halves, and root tip explants were cultured on 1.0x Murashige and Skoog (MS) medium containing 1.0, 2.0, 3.0, 4.0, or 5.0 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) or 1.0, 2.0, 3.0, 4.0, or 5.0 mg l(-1) 6-benzylaminopurine (BAP) plus 0.5 mg l(-1) alpha-naphthaleneacetic acid (NAA). The results indicated that root tip explants were most suitable for bulblet regeneration on 1.0x MS medium containing 5.0 mg l(-1) BAP plus 0.5 mg l(-1) NAA. All other explants failed to regenerate on different concentrations of BAP plus NAA, or on 2,4-D. The regenerated bulblets were acclimatised at 24 degrees +/- 1 degrees C and 80.0% relative humidity under growth chamber conditions, then transferred to pots containing peat-moss in a greenhouse. The results will be important for garlic breeders and researchers.", 
  "author": [
    {
      "family": "Kizil", 
      "given": " S."
    }, 
    {
      "family": "Icgil", 
      "given": " D. Y."
    }, 
    {
      "family": "Khawar", 
      "given": " K. M."
    }
  ], 
  "container_title": "JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY", 
  "id": "62511", 
  "issue": "4", 
  "issued": {
    "date-parts": [
      [
        2014, 
        1, 
        1
      ]
    ]
  }, 
  "page": "408-414", 
  "title": "Improved in vitro regeneration and propagation of Tunceli garlic (Allium tuncelianum L.)", 
  "type": "article-journal", 
  "volume": "89"
}
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