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Improved in vitro regeneration and propagation of Tunceli garlic (Allium tuncelianum L.)

Kizil, S.; Icgil, D. Y.; Khawar, K. M.


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{
  "@context": "https://schema.org/", 
  "@id": 62511, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Dicle Univ, Dept Field Crops, Fac Agr, TR-21280 Diyarbakir, Turkey", 
      "name": "Kizil, S."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Dicle Univ, Dept Field Crops, Fac Agr, TR-21280 Diyarbakir, Turkey", 
      "name": "Icgil, D. Y."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ankara Univ, Dept Field Crops, Fac Agr, TR-06110 Ankara, Turkey", 
      "name": "Khawar, K. M."
    }
  ], 
  "datePublished": "2014-01-01", 
  "description": "Over-exploitation of endemic and threatened Tunceli garlic [Allium tuncelianum (Kollman) N. Ozhatay, B. Mathew & Siraneci] for household purposes has threatened the species and requires that a reliable, improved tissue culture protocol be developed for its conservation. Leaf tips, the middle portions of leaves, leaf bases, vertically-sectioned halved or quartered bulbs, horizontally-sectioned upper and lower bulb halves, and root tip explants were cultured on 1.0x Murashige and Skoog (MS) medium containing 1.0, 2.0, 3.0, 4.0, or 5.0 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) or 1.0, 2.0, 3.0, 4.0, or 5.0 mg l(-1) 6-benzylaminopurine (BAP) plus 0.5 mg l(-1) alpha-naphthaleneacetic acid (NAA). The results indicated that root tip explants were most suitable for bulblet regeneration on 1.0x MS medium containing 5.0 mg l(-1) BAP plus 0.5 mg l(-1) NAA. All other explants failed to regenerate on different concentrations of BAP plus NAA, or on 2,4-D. The regenerated bulblets were acclimatised at 24 degrees +/- 1 degrees C and 80.0% relative humidity under growth chamber conditions, then transferred to pots containing peat-moss in a greenhouse. The results will be important for garlic breeders and researchers.", 
  "headline": "Improved in vitro regeneration and propagation of Tunceli garlic (Allium tuncelianum L.)", 
  "identifier": 62511, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Improved in vitro regeneration and propagation of Tunceli garlic (Allium tuncelianum L.)", 
  "url": "https://aperta.ulakbim.gov.tr/record/62511"
}
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