1 Ocak 2018 Dergi makalesi Açık Erişim

Alarcon-Martinez, Luis; Yilmaz-Ozcan, Sinem; Yemisci, Muge; Schallek, Jesse; Kilic, Kivilcim; Can, Alp; Di Polo, Adriana; Dalkara, Turgay

Dublin Core

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  <dc:creator>Alarcon-Martinez, Luis</dc:creator>
  <dc:creator>Yilmaz-Ozcan, Sinem</dc:creator>
  <dc:creator>Yemisci, Muge</dc:creator>
  <dc:creator>Schallek, Jesse</dc:creator>
  <dc:creator>Kilic, Kivilcim</dc:creator>
  <dc:creator>Can, Alp</dc:creator>
  <dc:creator>Di Polo, Adriana</dc:creator>
  <dc:creator>Dalkara, Turgay</dc:creator>
  <dc:date>2018-01-01</dc:date>
  <dc:description>Recent evidence suggests that capillary pericytes are contractile and play a crucial role in the regulation of microcirculation. However, failure to detect components of the contractile apparatus in capillary pericytes, most notably alpha-smooth muscle actin (alpha-SMA), has questioned these findings. Using strategies that allow rapid filamentous-actin (F-actin) fixation (i.e. snap freeze fixation with methanol at -20 degrees C) or prevent F-actin depolymerization (i.e. with F-actin stabilizing agents), we demonstrate that pericytes on mouse retinal capillaries, including those in intermediate and deeper plexus, express alpha-SMA. Junctional pericytes were more frequently alpha-SMA-positive relative to pericytes on linear capillary segments. Intravitreal administration of short interfering RNA (alpha-SMA-siRNA) suppressed alpha-SMA expression preferentially in high order branch capillary pericytes, confirming the existence of a smaller pool of alpha-SMA in distal capillary pericytes that is quickly lost by depolymerization. We conclude that capillary pericytes do express alpha-SMA, which rapidly depolymerizes during tissue fixation thus evading detection by immunolabeling.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/35445</dc:identifier>
  <dc:identifier>oai:zenodo.org:35445</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>ELIFE 7</dc:source>
  <dc:title>Capillary pericytes express alpha-smooth muscle actin, which requires prevention of filamentous-actin depolymerization for detection</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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