1 Ocak 2018 Dergi makalesi Açık Erişim

Alarcon-Martinez, Luis; Yilmaz-Ozcan, Sinem; Yemisci, Muge; Schallek, Jesse; Kilic, Kivilcim; Can, Alp; Di Polo, Adriana; Dalkara, Turgay

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/35445</identifier>
  <creators>
    <creator>
      <creatorName>Alarcon-Martinez, Luis</creatorName>
      <givenName>Luis</givenName>
      <familyName>Alarcon-Martinez</familyName>
    </creator>
    <creator>
      <creatorName>Yilmaz-Ozcan, Sinem</creatorName>
      <givenName>Sinem</givenName>
      <familyName>Yilmaz-Ozcan</familyName>
      <affiliation>Hacettepe Univ, Inst Neurol Sci &amp; Psychiat, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Yemisci, Muge</creatorName>
      <givenName>Muge</givenName>
      <familyName>Yemisci</familyName>
    </creator>
    <creator>
      <creatorName>Schallek, Jesse</creatorName>
      <givenName>Jesse</givenName>
      <familyName>Schallek</familyName>
    </creator>
    <creator>
      <creatorName>Kilic, Kivilcim</creatorName>
      <givenName>Kivilcim</givenName>
      <familyName>Kilic</familyName>
      <affiliation>Hacettepe Univ, Inst Neurol Sci &amp; Psychiat, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Can, Alp</creatorName>
      <givenName>Alp</givenName>
      <familyName>Can</familyName>
      <affiliation>Ankara Univ, Dept Histol &amp; Embryol, Sch Med, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Di Polo, Adriana</creatorName>
      <givenName>Adriana</givenName>
      <familyName>Di Polo</familyName>
    </creator>
    <creator>
      <creatorName>Dalkara, Turgay</creatorName>
      <givenName>Turgay</givenName>
      <familyName>Dalkara</familyName>
    </creator>
  </creators>
  <titles>
    <title>Capillary Pericytes Express Alpha-Smooth Muscle Actin, Which Requires Prevention Of Filamentous-Actin Depolymerization For Detection</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2018</publicationYear>
  <dates>
    <date dateType="Issued">2018-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/35445</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.7554/eLife.34861</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Recent evidence suggests that capillary pericytes are contractile and play a crucial role in the regulation of microcirculation. However, failure to detect components of the contractile apparatus in capillary pericytes, most notably alpha-smooth muscle actin (alpha-SMA), has questioned these findings. Using strategies that allow rapid filamentous-actin (F-actin) fixation (i.e. snap freeze fixation with methanol at -20 degrees C) or prevent F-actin depolymerization (i.e. with F-actin stabilizing agents), we demonstrate that pericytes on mouse retinal capillaries, including those in intermediate and deeper plexus, express alpha-SMA. Junctional pericytes were more frequently alpha-SMA-positive relative to pericytes on linear capillary segments. Intravitreal administration of short interfering RNA (alpha-SMA-siRNA) suppressed alpha-SMA expression preferentially in high order branch capillary pericytes, confirming the existence of a smaller pool of alpha-SMA in distal capillary pericytes that is quickly lost by depolymerization. We conclude that capillary pericytes do express alpha-SMA, which rapidly depolymerizes during tissue fixation thus evading detection by immunolabeling.</description>
  </descriptions>
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