1 Ocak 2018 Dergi makalesi Açık Erişim

Alarcon-Martinez, Luis; Yilmaz-Ozcan, Sinem; Yemisci, Muge; Schallek, Jesse; Kilic, Kivilcim; Can, Alp; Di Polo, Adriana; Dalkara, Turgay

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{
  "DOI": "10.7554/eLife.34861", 
  "abstract": "Recent evidence suggests that capillary pericytes are contractile and play a crucial role in the regulation of microcirculation. However, failure to detect components of the contractile apparatus in capillary pericytes, most notably alpha-smooth muscle actin (alpha-SMA), has questioned these findings. Using strategies that allow rapid filamentous-actin (F-actin) fixation (i.e. snap freeze fixation with methanol at -20 degrees C) or prevent F-actin depolymerization (i.e. with F-actin stabilizing agents), we demonstrate that pericytes on mouse retinal capillaries, including those in intermediate and deeper plexus, express alpha-SMA. Junctional pericytes were more frequently alpha-SMA-positive relative to pericytes on linear capillary segments. Intravitreal administration of short interfering RNA (alpha-SMA-siRNA) suppressed alpha-SMA expression preferentially in high order branch capillary pericytes, confirming the existence of a smaller pool of alpha-SMA in distal capillary pericytes that is quickly lost by depolymerization. We conclude that capillary pericytes do express alpha-SMA, which rapidly depolymerizes during tissue fixation thus evading detection by immunolabeling.", 
  "author": [
    {
      "family": "Alarcon-Martinez", 
      "given": " Luis"
    }, 
    {
      "family": "Yilmaz-Ozcan", 
      "given": " Sinem"
    }, 
    {
      "family": "Yemisci", 
      "given": " Muge"
    }, 
    {
      "family": "Schallek", 
      "given": " Jesse"
    }, 
    {
      "family": "Kilic", 
      "given": " Kivilcim"
    }, 
    {
      "family": "Can", 
      "given": " Alp"
    }, 
    {
      "family": "Di Polo", 
      "given": " Adriana"
    }, 
    {
      "family": "Dalkara", 
      "given": " Turgay"
    }
  ], 
  "container_title": "ELIFE", 
  "id": "35445", 
  "issued": {
    "date-parts": [
      [
        2018, 
        1, 
        1
      ]
    ]
  }, 
  "title": "Capillary pericytes express alpha-smooth muscle actin, which requires prevention of filamentous-actin depolymerization for detection", 
  "type": "article-journal", 
  "volume": "7"
}