1 Ocak 2018 Dergi makalesi Açık Erişim

Alarcon-Martinez, Luis; Yilmaz-Ozcan, Sinem; Yemisci, Muge; Schallek, Jesse; Kilic, Kivilcim; Can, Alp; Di Polo, Adriana; Dalkara, Turgay

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  "@context": "https://schema.org/", 
  "@id": 35445, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "name": "Alarcon-Martinez, Luis"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Hacettepe Univ, Inst Neurol Sci & Psychiat, Ankara, Turkey", 
      "name": "Yilmaz-Ozcan, Sinem"
    }, 
    {
      "@type": "Person", 
      "name": "Yemisci, Muge"
    }, 
    {
      "@type": "Person", 
      "name": "Schallek, Jesse"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Hacettepe Univ, Inst Neurol Sci & Psychiat, Ankara, Turkey", 
      "name": "Kilic, Kivilcim"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ankara Univ, Dept Histol & Embryol, Sch Med, Ankara, Turkey", 
      "name": "Can, Alp"
    }, 
    {
      "@type": "Person", 
      "name": "Di Polo, Adriana"
    }, 
    {
      "@type": "Person", 
      "name": "Dalkara, Turgay"
    }
  ], 
  "datePublished": "2018-01-01", 
  "description": "Recent evidence suggests that capillary pericytes are contractile and play a crucial role in the regulation of microcirculation. However, failure to detect components of the contractile apparatus in capillary pericytes, most notably alpha-smooth muscle actin (alpha-SMA), has questioned these findings. Using strategies that allow rapid filamentous-actin (F-actin) fixation (i.e. snap freeze fixation with methanol at -20 degrees C) or prevent F-actin depolymerization (i.e. with F-actin stabilizing agents), we demonstrate that pericytes on mouse retinal capillaries, including those in intermediate and deeper plexus, express alpha-SMA. Junctional pericytes were more frequently alpha-SMA-positive relative to pericytes on linear capillary segments. Intravitreal administration of short interfering RNA (alpha-SMA-siRNA) suppressed alpha-SMA expression preferentially in high order branch capillary pericytes, confirming the existence of a smaller pool of alpha-SMA in distal capillary pericytes that is quickly lost by depolymerization. We conclude that capillary pericytes do express alpha-SMA, which rapidly depolymerizes during tissue fixation thus evading detection by immunolabeling.", 
  "headline": "Capillary pericytes express alpha-smooth muscle actin, which requires prevention of filamentous-actin depolymerization for detection", 
  "identifier": 35445, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Capillary pericytes express alpha-smooth muscle actin, which requires prevention of filamentous-actin depolymerization for detection", 
  "url": "https://aperta.ulakbim.gov.tr/record/35445"
}