1 Ocak 2023 Dergi makalesi Açık Erişim

Ercelik, Melis; Tunca, Berrin; Ak Aksoy, Secil; Tekin, Cagla; Tezcan, Gulcin

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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Ercelik, Melis</dc:creator>
  <dc:creator>Tunca, Berrin</dc:creator>
  <dc:creator>Ak Aksoy, Secil</dc:creator>
  <dc:creator>Tekin, Cagla</dc:creator>
  <dc:creator>Tezcan, Gulcin</dc:creator>
  <dc:date>2023-01-01</dc:date>
  <dc:description>Objectives: The purpose of this study was to investigate the effect of Olea europaea L. leaf extract (OLE) on senescence and senescence-associated secretory phenotype (SASP) caused by temozolomide (TMZ) in glioblastoma (GB).Materials and Methods: A senescence beta-galactosidase assay and a colony formation assay were used to determine the effects of OLE, TMZ, and OLE + TMZ on the cellular senescence and aggressiveness of GB cell lines T98G and U87MG. mRNA expression levels of p53, a senescence factor, interleukin (IL)-6, matrix metalloproteinases (MMP)-9, and nuclear factor kappa B1 (NF-kappa B1) as SASP factors and Bcl-2 and Bax as senolytic markers were assessed using quantitative reverse transcription-real-time polymerase chain reaction. Cells were double-stained with acridine orange and propidium iodide to observe the cell morphology.Results: TMZ increased the senescence rate of GB cells (p&lt;0.001). Besides, OLE + TMZ reduced the proportion of senescent cells (p&lt;0.001) and their capability to form colonies compared to TMZ-only-treated cells. Additionally, OLE + TMZ co-treatment elevated the mRNA expression levels of MMP-9, IL-6, NF-kappa B1, p53, and the Bax/Bcl-2 ratio compared to TMZ-only treatment. Especially in U87MG cells, involvement of OLE in TMZ treatments increased more than six times in the Bax/Bcl-2 ratio compared to TMZ-only, which induced the apoptosis-like morphological features (p&lt;0.0001).Conclusion: Collectively, our findings presented the inhibitory effect of OLE on TMZ-mediated SASP-factor production in GB and, accordingly, its potential contribution to elongate the time of recurrence.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/273490</dc:identifier>
  <dc:identifier>oai:aperta.ulakbim.gov.tr:273490</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>TURKISH JOURNAL OF PHARMACEUTICAL SCIENCES 20(2) 10</dc:source>
  <dc:title>&lt;i&gt;Olea&lt;/i&gt;&lt;i&gt; europaea&lt;/i&gt; L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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