1 Ocak 2023 Dergi makalesi Açık Erişim

Ercelik, Melis; Tunca, Berrin; Ak Aksoy, Secil; Tekin, Cagla; Tezcan, Gulcin

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/273490</identifier>
  <creators>
    <creator>
      <creatorName>Ercelik, Melis</creatorName>
      <givenName>Melis</givenName>
      <familyName>Ercelik</familyName>
      <affiliation>Bursa Uludag Univ, Fac Med, Dept Med Biol, Bursa, Turkiye</affiliation>
    </creator>
    <creator>
      <creatorName>Tunca, Berrin</creatorName>
      <givenName>Berrin</givenName>
      <familyName>Tunca</familyName>
      <affiliation>Bursa Uludag Univ, Fac Med, Dept Med Biol, Bursa, Turkiye</affiliation>
    </creator>
    <creator>
      <creatorName>Ak Aksoy, Secil</creatorName>
      <givenName>Secil</givenName>
      <familyName>Ak Aksoy</familyName>
      <affiliation>Bursa Uludag Univ, Inegol Vocat Sch, Bursa, Turkiye</affiliation>
    </creator>
    <creator>
      <creatorName>Tekin, Cagla</creatorName>
      <givenName>Cagla</givenName>
      <familyName>Tekin</familyName>
      <affiliation>Bursa Uludag Univ, Fac Med, Dept Med Biol, Bursa, Turkiye</affiliation>
    </creator>
    <creator>
      <creatorName>Tezcan, Gulcin</creatorName>
      <givenName>Gulcin</givenName>
      <familyName>Tezcan</familyName>
      <affiliation>Bursa Uludag Univ, Fac Dent, Dept Fundamental Sci, Bursa, Turkiye</affiliation>
    </creator>
  </creators>
  <titles>
    <title>&lt;I&gt;Olea&lt;/I&gt;&lt;I&gt; Europaea&lt;/I&gt; L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype In Glioblastoma</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2023</publicationYear>
  <dates>
    <date dateType="Issued">2023-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/273490</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.4274/tjps.galenos.2022.57639</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">&lt;p&gt;Objectives: The purpose of this study was to investigate the effect of Olea europaea L. leaf extract (OLE) on senescence and senescence-associated secretory phenotype (SASP) caused by temozolomide (TMZ) in glioblastoma (GB).Materials and Methods: A senescence beta-galactosidase assay and a colony formation assay were used to determine the effects of OLE, TMZ, and OLE + TMZ on the cellular senescence and aggressiveness of GB cell lines T98G and U87MG. mRNA expression levels of p53, a senescence factor, interleukin (IL)-6, matrix metalloproteinases (MMP)-9, and nuclear factor kappa B1 (NF-kappa B1) as SASP factors and Bcl-2 and Bax as senolytic markers were assessed using quantitative reverse transcription-real-time polymerase chain reaction. Cells were double-stained with acridine orange and propidium iodide to observe the cell morphology.Results: TMZ increased the senescence rate of GB cells (p&amp;lt;0.001). Besides, OLE + TMZ reduced the proportion of senescent cells (p&amp;lt;0.001) and their capability to form colonies compared to TMZ-only-treated cells. Additionally, OLE + TMZ co-treatment elevated the mRNA expression levels of MMP-9, IL-6, NF-kappa B1, p53, and the Bax/Bcl-2 ratio compared to TMZ-only treatment. Especially in U87MG cells, involvement of OLE in TMZ treatments increased more than six times in the Bax/Bcl-2 ratio compared to TMZ-only, which induced the apoptosis-like morphological features (p&amp;lt;0.0001).Conclusion: Collectively, our findings presented the inhibitory effect of OLE on TMZ-mediated SASP-factor production in GB and, accordingly, its potential contribution to elongate the time of recurrence.&lt;/p&gt;</description>
  </descriptions>
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