1 Ocak 2023 Dergi makalesi Açık Erişim

Ercelik, Melis; Tunca, Berrin; Ak Aksoy, Secil; Tekin, Cagla; Tezcan, Gulcin

Citation Style Language JSON

{
  "DOI": "10.4274/tjps.galenos.2022.57639", 
  "abstract": "<p>Objectives: The purpose of this study was to investigate the effect of Olea europaea L. leaf extract (OLE) on senescence and senescence-associated secretory phenotype (SASP) caused by temozolomide (TMZ) in glioblastoma (GB).Materials and Methods: A senescence beta-galactosidase assay and a colony formation assay were used to determine the effects of OLE, TMZ, and OLE + TMZ on the cellular senescence and aggressiveness of GB cell lines T98G and U87MG. mRNA expression levels of p53, a senescence factor, interleukin (IL)-6, matrix metalloproteinases (MMP)-9, and nuclear factor kappa B1 (NF-kappa B1) as SASP factors and Bcl-2 and Bax as senolytic markers were assessed using quantitative reverse transcription-real-time polymerase chain reaction. Cells were double-stained with acridine orange and propidium iodide to observe the cell morphology.Results: TMZ increased the senescence rate of GB cells (p&lt;0.001). Besides, OLE + TMZ reduced the proportion of senescent cells (p&lt;0.001) and their capability to form colonies compared to TMZ-only-treated cells. Additionally, OLE + TMZ co-treatment elevated the mRNA expression levels of MMP-9, IL-6, NF-kappa B1, p53, and the Bax/Bcl-2 ratio compared to TMZ-only treatment. Especially in U87MG cells, involvement of OLE in TMZ treatments increased more than six times in the Bax/Bcl-2 ratio compared to TMZ-only, which induced the apoptosis-like morphological features (p&lt;0.0001).Conclusion: Collectively, our findings presented the inhibitory effect of OLE on TMZ-mediated SASP-factor production in GB and, accordingly, its potential contribution to elongate the time of recurrence.</p>", 
  "author": [
    {
      "family": "Ercelik", 
      "given": " Melis"
    }, 
    {
      "family": "Tunca", 
      "given": " Berrin"
    }, 
    {
      "family": "Ak Aksoy", 
      "given": " Secil"
    }, 
    {
      "family": "Tekin", 
      "given": " Cagla"
    }, 
    {
      "family": "Tezcan", 
      "given": " Gulcin"
    }
  ], 
  "container_title": "TURKISH JOURNAL OF PHARMACEUTICAL SCIENCES", 
  "id": "273490", 
  "issue": "2", 
  "issued": {
    "date-parts": [
      [
        2023, 
        1, 
        1
      ]
    ]
  }, 
  "page": "10", 
  "title": "<i>Olea</i><i> europaea</i> L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma", 
  "type": "article-journal", 
  "volume": "20"
}