Dergi makalesi Açık Erişim
Ercelik, Melis; Tunca, Berrin; Ak Aksoy, Secil; Tekin, Cagla; Tezcan, Gulcin
{ "conceptrecid": "273489", "created": "2024-06-08T07:01:07.939533+00:00", "doi": "10.4274/tjps.galenos.2022.57639", "files": [ { "bucket": "d1ed7852-b702-4efc-bb4e-b8061d3c5eb6", "checksum": "md5:8325397abe968679166affd35dd85258", "key": "bib-29608dab-145c-4a27-b428-21708a20c34e.txt", "links": { "self": "https://aperta.ulakbim.gov.tr/api/files/d1ed7852-b702-4efc-bb4e-b8061d3c5eb6/bib-29608dab-145c-4a27-b428-21708a20c34e.txt" }, "size": 266, "type": "txt" } ], "id": 273490, "links": { "badge": "https://aperta.ulakbim.gov.tr/badge/doi/10.4274/tjps.galenos.2022.57639.svg", "bucket": "https://aperta.ulakbim.gov.tr/api/files/d1ed7852-b702-4efc-bb4e-b8061d3c5eb6", "doi": "https://doi.org/10.4274/tjps.galenos.2022.57639", "html": "https://aperta.ulakbim.gov.tr/record/273490", "latest": "https://aperta.ulakbim.gov.tr/api/records/273490", "latest_html": "https://aperta.ulakbim.gov.tr/record/273490" }, "metadata": { "access_right": "open", "access_right_category": "success", "communities": [ { "id": "tubitak-destekli-proje-yayinlari" } ], "creators": [ { "affiliation": "Bursa Uludag Univ, Fac Med, Dept Med Biol, Bursa, Turkiye", "name": "Ercelik, Melis" }, { "affiliation": "Bursa Uludag Univ, Fac Med, Dept Med Biol, Bursa, Turkiye", "name": "Tunca, Berrin" }, { "affiliation": "Bursa Uludag Univ, Inegol Vocat Sch, Bursa, Turkiye", "name": "Ak Aksoy, Secil" }, { "affiliation": "Bursa Uludag Univ, Fac Med, Dept Med Biol, Bursa, Turkiye", "name": "Tekin, Cagla" }, { "affiliation": "Bursa Uludag Univ, Fac Dent, Dept Fundamental Sci, Bursa, Turkiye", "name": "Tezcan, Gulcin" } ], "description": "<p>Objectives: The purpose of this study was to investigate the effect of Olea europaea L. leaf extract (OLE) on senescence and senescence-associated secretory phenotype (SASP) caused by temozolomide (TMZ) in glioblastoma (GB).Materials and Methods: A senescence beta-galactosidase assay and a colony formation assay were used to determine the effects of OLE, TMZ, and OLE + TMZ on the cellular senescence and aggressiveness of GB cell lines T98G and U87MG. mRNA expression levels of p53, a senescence factor, interleukin (IL)-6, matrix metalloproteinases (MMP)-9, and nuclear factor kappa B1 (NF-kappa B1) as SASP factors and Bcl-2 and Bax as senolytic markers were assessed using quantitative reverse transcription-real-time polymerase chain reaction. Cells were double-stained with acridine orange and propidium iodide to observe the cell morphology.Results: TMZ increased the senescence rate of GB cells (p<0.001). Besides, OLE + TMZ reduced the proportion of senescent cells (p<0.001) and their capability to form colonies compared to TMZ-only-treated cells. Additionally, OLE + TMZ co-treatment elevated the mRNA expression levels of MMP-9, IL-6, NF-kappa B1, p53, and the Bax/Bcl-2 ratio compared to TMZ-only treatment. Especially in U87MG cells, involvement of OLE in TMZ treatments increased more than six times in the Bax/Bcl-2 ratio compared to TMZ-only, which induced the apoptosis-like morphological features (p<0.0001).Conclusion: Collectively, our findings presented the inhibitory effect of OLE on TMZ-mediated SASP-factor production in GB and, accordingly, its potential contribution to elongate the time of recurrence.</p>", "doi": "10.4274/tjps.galenos.2022.57639", "has_grant": false, "journal": { "issue": "2", "pages": "10", "title": "TURKISH JOURNAL OF PHARMACEUTICAL SCIENCES", "volume": "20" }, "license": { "id": "cc-by" }, "publication_date": "2023-01-01", "relations": { "version": [ { "count": 1, "index": 0, "is_last": true, "last_child": { "pid_type": "recid", "pid_value": "273490" }, "parent": { "pid_type": "recid", "pid_value": "273489" } } ] }, "resource_type": { "subtype": "article", "title": "Dergi makalesi", "type": "publication" }, "science_branches": [ "Di\u011fer" ], "title": "<i>Olea</i><i> europaea</i> L. Leaf Extract Attenuates Temozolomide-Induced Senescence-Associated Secretion Phenotype in Glioblastoma" }, "owners": [ 1 ], "revision": 1, "stats": { "downloads": 0.0, "unique_downloads": 0.0, "unique_views": 0.0, "version_downloads": 0.0, "version_unique_downloads": 0.0, "version_unique_views": 0.0, "version_views": 0.0, "version_volume": 0.0, "views": 0.0, "volume": 0.0 }, "updated": "2024-06-08T07:01:07.975317+00:00" }
Görüntülenme | 0 |
İndirme | 0 |
Veri hacmi | 0 Bytes |
Tekil görüntülenme | 0 |
Tekil indirme | 0 |