1 Ocak 2021 Dergi makalesi Açık Erişim

Guelce-Iz, Sultan; Elaldi, Nazif; Can, Hueseyin; Sahar, Esra Atalay; Karakavuk, Muhammet; Gul, Aytul; Kumoglu, Gizem Ors; Doskaya, Aysu Degirmenci; Gueruez, Adnan Yueksel; Ozdarendeli, Aykut; Felgner, Philip Louis; Davies, Huw; Doskaya, Mert

Dublin Core

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  <dc:creator>Guelce-Iz, Sultan</dc:creator>
  <dc:creator>Elaldi, Nazif</dc:creator>
  <dc:creator>Can, Hueseyin</dc:creator>
  <dc:creator>Sahar, Esra Atalay</dc:creator>
  <dc:creator>Karakavuk, Muhammet</dc:creator>
  <dc:creator>Gul, Aytul</dc:creator>
  <dc:creator>Kumoglu, Gizem Ors</dc:creator>
  <dc:creator>Doskaya, Aysu Degirmenci</dc:creator>
  <dc:creator>Gueruez, Adnan Yueksel</dc:creator>
  <dc:creator>Ozdarendeli, Aykut</dc:creator>
  <dc:creator>Felgner, Philip Louis</dc:creator>
  <dc:creator>Davies, Huw</dc:creator>
  <dc:creator>Doskaya, Mert</dc:creator>
  <dc:date>2021-01-01</dc:date>
  <dc:description>Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral infection caused by Crimean-Congo hemorrhagic fever virus (CCHFV). Serological screening of CCHF is important and current ELISA use antigens prepared from virus which is expensive due to requirement of high bio-containment facilities. In this study, we aimed to develop a new recombinant ELISA. For this purpose, CCHFV genome were expressed as 13 proteins in E. coli and among them abundantly purified recombinant Nucleocapsid protein (rNP) and Mucin-like variable domain (rMLD) were used as antigen in ELISA (Rec-ELISA). Rec-ELISA using rNP, rMLD and a combination of both (rNP/rMLD) were probed with acute (n=64; collected between days 1 and 7 after onset of symptoms), convalescent (n=35; collected 8 days after onset of symptoms), consecutive sera (n=25) of confirmed CCHF cases and control sera (n=43). The sensitivity and specificity of Rec-ELISA using rNP/rMLD were 73% and 98% in acute cases and 97% and 98% in convalescent cases. The median interquartile absorbance value to discriminate the acute and convalescent phases of CCHF was significantly higher with ELISA using rNP/rMLD (P&lt;0.0001) compared to rNP (P&gt;0.05) and rMLD (P=0.001). These results indicate that the Rec-ELISA using rNP/rMLD may be very useful to diagnose convalescent CCHF cases especially in field studies.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/238278</dc:identifier>
  <dc:identifier>oai:aperta.ulakbim.gov.tr:238278</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>SCIENTIFIC REPORTS 11(1)</dc:source>
  <dc:title>Development of a novel recombinant ELISA for the detection of Crimean-Congo hemorrhagic fever virus IgG antibodies</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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