1 Ocak 2021 Dergi makalesi Açık Erişim

Guelce-Iz, Sultan; Elaldi, Nazif; Can, Hueseyin; Sahar, Esra Atalay; Karakavuk, Muhammet; Gul, Aytul; Kumoglu, Gizem Ors; Doskaya, Aysu Degirmenci; Gueruez, Adnan Yueksel; Ozdarendeli, Aykut; Felgner, Philip Louis; Davies, Huw; Doskaya, Mert

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/238278</identifier>
  <creators>
    <creator>
      <creatorName>Guelce-Iz, Sultan</creatorName>
      <givenName>Sultan</givenName>
      <familyName>Guelce-Iz</familyName>
    </creator>
    <creator>
      <creatorName>Elaldi, Nazif</creatorName>
      <givenName>Nazif</givenName>
      <familyName>Elaldi</familyName>
      <affiliation>Sivas Cumhuriyet Univ, Dept Infect Dis &amp; Clin Microbiol, Fac Med, Sivas, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Can, Hueseyin</creatorName>
      <givenName>Hueseyin</givenName>
      <familyName>Can</familyName>
      <affiliation>Ege Univ, Mol Biol Sect, Dept Biol, Fac Sci, Izmir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Sahar, Esra Atalay</creatorName>
      <givenName>Esra Atalay</givenName>
      <familyName>Sahar</familyName>
      <affiliation>Ege Univ, Dept Biotechnol, Fac Engn, Izmir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Karakavuk, Muhammet</creatorName>
      <givenName>Muhammet</givenName>
      <familyName>Karakavuk</familyName>
      <affiliation>Ege Univ, Dept Parasitol, Fac Med, Izmir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Gul, Aytul</creatorName>
      <givenName>Aytul</givenName>
      <familyName>Gul</familyName>
      <affiliation>Ege Univ, Dept Bioengn, Fac Engn, Izmir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Kumoglu, Gizem Ors</creatorName>
      <givenName>Gizem Ors</givenName>
      <familyName>Kumoglu</familyName>
      <affiliation>Ege Univ, Dept Bioengn, Fac Engn, Izmir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Doskaya, Aysu Degirmenci</creatorName>
      <givenName>Aysu Degirmenci</givenName>
      <familyName>Doskaya</familyName>
    </creator>
    <creator>
      <creatorName>Gueruez, Adnan Yueksel</creatorName>
      <givenName>Adnan Yueksel</givenName>
      <familyName>Gueruez</familyName>
      <affiliation>Ege Univ, Dept Parasitol, Fac Med, Izmir, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ozdarendeli, Aykut</creatorName>
      <givenName>Aykut</givenName>
      <familyName>Ozdarendeli</familyName>
      <affiliation>Erciyes Univ, Dept Med Microbiol, Fac Med, Kayseri, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Felgner, Philip Louis</creatorName>
      <givenName>Philip Louis</givenName>
      <familyName>Felgner</familyName>
      <affiliation>Univ Calif Irvine, Dept Physiol &amp; Biophys, Vaccine Res &amp; Dev Ctr, Irvine, CA 92717 USA</affiliation>
    </creator>
    <creator>
      <creatorName>Davies, Huw</creatorName>
      <givenName>Huw</givenName>
      <familyName>Davies</familyName>
      <affiliation>Univ Calif Irvine, Dept Physiol &amp; Biophys, Vaccine Res &amp; Dev Ctr, Irvine, CA 92717 USA</affiliation>
    </creator>
    <creator>
      <creatorName>Doskaya, Mert</creatorName>
      <givenName>Mert</givenName>
      <familyName>Doskaya</familyName>
      <affiliation>Ege Univ, Dept Parasitol, Fac Med, Izmir, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Development Of A Novel Recombinant Elisa For The Detection Of Crimean-Congo Hemorrhagic Fever Virus Igg Antibodies</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2021</publicationYear>
  <dates>
    <date dateType="Issued">2021-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/238278</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1038/s41598-021-85323-1</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral infection caused by Crimean-Congo hemorrhagic fever virus (CCHFV). Serological screening of CCHF is important and current ELISA use antigens prepared from virus which is expensive due to requirement of high bio-containment facilities. In this study, we aimed to develop a new recombinant ELISA. For this purpose, CCHFV genome were expressed as 13 proteins in E. coli and among them abundantly purified recombinant Nucleocapsid protein (rNP) and Mucin-like variable domain (rMLD) were used as antigen in ELISA (Rec-ELISA). Rec-ELISA using rNP, rMLD and a combination of both (rNP/rMLD) were probed with acute (n=64; collected between days 1 and 7 after onset of symptoms), convalescent (n=35; collected 8 days after onset of symptoms), consecutive sera (n=25) of confirmed CCHF cases and control sera (n=43). The sensitivity and specificity of Rec-ELISA using rNP/rMLD were 73% and 98% in acute cases and 97% and 98% in convalescent cases. The median interquartile absorbance value to discriminate the acute and convalescent phases of CCHF was significantly higher with ELISA using rNP/rMLD (P&amp;lt;0.0001) compared to rNP (P&amp;gt;0.05) and rMLD (P=0.001). These results indicate that the Rec-ELISA using rNP/rMLD may be very useful to diagnose convalescent CCHF cases especially in field studies.</description>
  </descriptions>
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