1 Ocak 2021 Dergi makalesi Açık Erişim

Guelce-Iz, Sultan; Elaldi, Nazif; Can, Hueseyin; Sahar, Esra Atalay; Karakavuk, Muhammet; Gul, Aytul; Kumoglu, Gizem Ors; Doskaya, Aysu Degirmenci; Gueruez, Adnan Yueksel; Ozdarendeli, Aykut; Felgner, Philip Louis; Davies, Huw; Doskaya, Mert

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        "id": "tubitak-destekli-proje-yayinlari"
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    "creators": [
      {
        "name": "Guelce-Iz, Sultan"
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      {
        "affiliation": "Sivas Cumhuriyet Univ, Dept Infect Dis & Clin Microbiol, Fac Med, Sivas, Turkey", 
        "name": "Elaldi, Nazif"
      }, 
      {
        "affiliation": "Ege Univ, Mol Biol Sect, Dept Biol, Fac Sci, Izmir, Turkey", 
        "name": "Can, Hueseyin"
      }, 
      {
        "affiliation": "Ege Univ, Dept Biotechnol, Fac Engn, Izmir, Turkey", 
        "name": "Sahar, Esra Atalay"
      }, 
      {
        "affiliation": "Ege Univ, Dept Parasitol, Fac Med, Izmir, Turkey", 
        "name": "Karakavuk, Muhammet"
      }, 
      {
        "affiliation": "Ege Univ, Dept Bioengn, Fac Engn, Izmir, Turkey", 
        "name": "Gul, Aytul"
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      {
        "affiliation": "Ege Univ, Dept Bioengn, Fac Engn, Izmir, Turkey", 
        "name": "Kumoglu, Gizem Ors"
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      {
        "name": "Doskaya, Aysu Degirmenci"
      }, 
      {
        "affiliation": "Ege Univ, Dept Parasitol, Fac Med, Izmir, Turkey", 
        "name": "Gueruez, Adnan Yueksel"
      }, 
      {
        "affiliation": "Erciyes Univ, Dept Med Microbiol, Fac Med, Kayseri, Turkey", 
        "name": "Ozdarendeli, Aykut"
      }, 
      {
        "affiliation": "Univ Calif Irvine, Dept Physiol & Biophys, Vaccine Res & Dev Ctr, Irvine, CA 92717 USA", 
        "name": "Felgner, Philip Louis"
      }, 
      {
        "affiliation": "Univ Calif Irvine, Dept Physiol & Biophys, Vaccine Res & Dev Ctr, Irvine, CA 92717 USA", 
        "name": "Davies, Huw"
      }, 
      {
        "affiliation": "Ege Univ, Dept Parasitol, Fac Med, Izmir, Turkey", 
        "name": "Doskaya, Mert"
      }
    ], 
    "description": "Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral infection caused by Crimean-Congo hemorrhagic fever virus (CCHFV). Serological screening of CCHF is important and current ELISA use antigens prepared from virus which is expensive due to requirement of high bio-containment facilities. In this study, we aimed to develop a new recombinant ELISA. For this purpose, CCHFV genome were expressed as 13 proteins in E. coli and among them abundantly purified recombinant Nucleocapsid protein (rNP) and Mucin-like variable domain (rMLD) were used as antigen in ELISA (Rec-ELISA). Rec-ELISA using rNP, rMLD and a combination of both (rNP/rMLD) were probed with acute (n=64; collected between days 1 and 7 after onset of symptoms), convalescent (n=35; collected 8 days after onset of symptoms), consecutive sera (n=25) of confirmed CCHF cases and control sera (n=43). The sensitivity and specificity of Rec-ELISA using rNP/rMLD were 73% and 98% in acute cases and 97% and 98% in convalescent cases. The median interquartile absorbance value to discriminate the acute and convalescent phases of CCHF was significantly higher with ELISA using rNP/rMLD (P<0.0001) compared to rNP (P>0.05) and rMLD (P=0.001). These results indicate that the Rec-ELISA using rNP/rMLD may be very useful to diagnose convalescent CCHF cases especially in field studies.", 
    "doi": "10.1038/s41598-021-85323-1", 
    "has_grant": false, 
    "journal": {
      "issue": "1", 
      "title": "SCIENTIFIC REPORTS", 
      "volume": "11"
    }, 
    "license": {
      "id": "cc-by"
    }, 
    "publication_date": "2021-01-01", 
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    "resource_type": {
      "subtype": "article", 
      "title": "Dergi makalesi", 
      "type": "publication"
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    "science_branches": [
      "Di\u011fer"
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    "title": "Development of a novel recombinant ELISA for the detection of Crimean-Congo hemorrhagic fever virus IgG antibodies"
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