1 Ocak 2021 Dergi makalesi Açık Erişim

Guelce-Iz, Sultan; Elaldi, Nazif; Can, Hueseyin; Sahar, Esra Atalay; Karakavuk, Muhammet; Gul, Aytul; Kumoglu, Gizem Ors; Doskaya, Aysu Degirmenci; Gueruez, Adnan Yueksel; Ozdarendeli, Aykut; Felgner, Philip Louis; Davies, Huw; Doskaya, Mert

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{
  "@context": "https://schema.org/", 
  "@id": 238278, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "name": "Guelce-Iz, Sultan"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Sivas Cumhuriyet Univ, Dept Infect Dis & Clin Microbiol, Fac Med, Sivas, Turkey", 
      "name": "Elaldi, Nazif"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ege Univ, Mol Biol Sect, Dept Biol, Fac Sci, Izmir, Turkey", 
      "name": "Can, Hueseyin"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ege Univ, Dept Biotechnol, Fac Engn, Izmir, Turkey", 
      "name": "Sahar, Esra Atalay"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ege Univ, Dept Parasitol, Fac Med, Izmir, Turkey", 
      "name": "Karakavuk, Muhammet"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ege Univ, Dept Bioengn, Fac Engn, Izmir, Turkey", 
      "name": "Gul, Aytul"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ege Univ, Dept Bioengn, Fac Engn, Izmir, Turkey", 
      "name": "Kumoglu, Gizem Ors"
    }, 
    {
      "@type": "Person", 
      "name": "Doskaya, Aysu Degirmenci"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ege Univ, Dept Parasitol, Fac Med, Izmir, Turkey", 
      "name": "Gueruez, Adnan Yueksel"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Erciyes Univ, Dept Med Microbiol, Fac Med, Kayseri, Turkey", 
      "name": "Ozdarendeli, Aykut"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Univ Calif Irvine, Dept Physiol & Biophys, Vaccine Res & Dev Ctr, Irvine, CA 92717 USA", 
      "name": "Felgner, Philip Louis"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Univ Calif Irvine, Dept Physiol & Biophys, Vaccine Res & Dev Ctr, Irvine, CA 92717 USA", 
      "name": "Davies, Huw"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ege Univ, Dept Parasitol, Fac Med, Izmir, Turkey", 
      "name": "Doskaya, Mert"
    }
  ], 
  "datePublished": "2021-01-01", 
  "description": "Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral infection caused by Crimean-Congo hemorrhagic fever virus (CCHFV). Serological screening of CCHF is important and current ELISA use antigens prepared from virus which is expensive due to requirement of high bio-containment facilities. In this study, we aimed to develop a new recombinant ELISA. For this purpose, CCHFV genome were expressed as 13 proteins in E. coli and among them abundantly purified recombinant Nucleocapsid protein (rNP) and Mucin-like variable domain (rMLD) were used as antigen in ELISA (Rec-ELISA). Rec-ELISA using rNP, rMLD and a combination of both (rNP/rMLD) were probed with acute (n=64; collected between days 1 and 7 after onset of symptoms), convalescent (n=35; collected 8 days after onset of symptoms), consecutive sera (n=25) of confirmed CCHF cases and control sera (n=43). The sensitivity and specificity of Rec-ELISA using rNP/rMLD were 73% and 98% in acute cases and 97% and 98% in convalescent cases. The median interquartile absorbance value to discriminate the acute and convalescent phases of CCHF was significantly higher with ELISA using rNP/rMLD (P<0.0001) compared to rNP (P>0.05) and rMLD (P=0.001). These results indicate that the Rec-ELISA using rNP/rMLD may be very useful to diagnose convalescent CCHF cases especially in field studies.", 
  "headline": "Development of a novel recombinant ELISA for the detection of Crimean-Congo hemorrhagic fever virus IgG antibodies", 
  "identifier": 238278, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Development of a novel recombinant ELISA for the detection of Crimean-Congo hemorrhagic fever virus IgG antibodies", 
  "url": "https://aperta.ulakbim.gov.tr/record/238278"
}