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Effect of co-substrate sorbitol different feeding strategies on human growth hormone production by recombinant Pichia pastoris

Calik, Pinar; Bozkurt, Bahar; Zerze, Gul H.; Inankur, Bahar; Bayraktar, Eda; Boy, Erdem; Orman, Mehmet Ali; Acik, Eda; Ozdamar, Tuncer H.


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{
  "DOI": "10.1002/jctb.4011", 
  "abstract": "BACKGROUNDEffects of co-substrate sorbitol different feeding strategies on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) were investigated by eight designed experiments grouped as: (i) fed-batch methanol feeding without the co-substrate; (ii) fed-batch methanol feeding with pulse sorbitol feeding; (iii) fed-batch methanol feeding together with fed-batch sorbitol feeding at t=0-15h, followed by fed-batch methanol feeding; and (iv) fed-batch methanol and sorbitol feeding at t=0-30h, followed with fed-batch methanol feeding.", 
  "author": [
    {
      "family": "Calik", 
      "given": " Pinar"
    }, 
    {
      "family": "Bozkurt", 
      "given": " Bahar"
    }, 
    {
      "family": "Zerze", 
      "given": " Gul H."
    }, 
    {
      "family": "Inankur", 
      "given": " Bahar"
    }, 
    {
      "family": "Bayraktar", 
      "given": " Eda"
    }, 
    {
      "family": "Boy", 
      "given": " Erdem"
    }, 
    {
      "family": "Orman", 
      "given": " Mehmet Ali"
    }, 
    {
      "family": "Acik", 
      "given": " Eda"
    }, 
    {
      "family": "Ozdamar", 
      "given": " Tuncer H."
    }
  ], 
  "container_title": "JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY", 
  "id": "17743", 
  "issue": "9", 
  "issued": {
    "date-parts": [
      [
        2013, 
        1, 
        1
      ]
    ]
  }, 
  "page": "1631-1640", 
  "title": "Effect of co-substrate sorbitol different feeding strategies on human growth hormone production by recombinant Pichia pastoris", 
  "type": "article-journal", 
  "volume": "88"
}
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