Dergi makalesi Açık Erişim

Effect of co-substrate sorbitol different feeding strategies on human growth hormone production by recombinant Pichia pastoris

2013    Calik, Pinar; Bozkurt, Bahar; Zerze, Gul H.; Inankur, Bahar; Bayraktar, Eda; Boy, Erdem; Orman, Mehmet Ali; Acik, Eda; Ozdamar, Tuncer H.

BACKGROUNDEffects of co-substrate sorbitol different feeding strategies on recombinant human growth hormone (rhGH) production by Pichia pastoris hGH-Mut(+) were investigated by eight designed experiments grouped as: (i) fed-batch methanol feeding without the co-substrate; (ii) fed-batch methanol feeding with pulse sorbitol feeding; (iii) fed-batch methanol feeding together with fed-batch sorbitol feeding at t=0-15h, followed by fed-batch methanol feeding; and (iv) fed-batch methanol and sorbitol feeding at t=0-30h, followed with fed-batch methanol feeding.