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Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg x Sultani progeny

Akkurt, Murat; Cakir, Atilla; Shidfar, Mina; Mutaf, Filiz; Soylemezoglu, Gokhan


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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Akkurt, Murat</dc:creator>
  <dc:creator>Cakir, Atilla</dc:creator>
  <dc:creator>Shidfar, Mina</dc:creator>
  <dc:creator>Mutaf, Filiz</dc:creator>
  <dc:creator>Soylemezoglu, Gokhan</dc:creator>
  <dc:date>2013-01-01</dc:date>
  <dc:description>The seedlessness-related molecular markers VMC7f2, SCC8, and SCF27 were used for the early selection of seedless individuals in the Muscat of Hamburg (seeded) x Sultani (seedless) progeny of 314 F1 hybrids. The DNA from F1 hybrids was amplified by polymerase chain reaction (PCR) using 3 markers. After digestion of the SCC8 marker PCR amplification products by restriction enzyme Bgl II, 72 individuals showed homozygous SCC8+/SCC8+ alleles at the seed development inhibitor locus, 76 individuals of the progeny amplified with the SCF27 marker produced 2.0-kb bands, and 118 individuals with seedlessness-related 198-bp alleles amplified with the VMC7f2 marker were selected as seedless grapevine candidates. Based on marker-assisted selection, 190 F1 hybrids were designated as seedless grapevine candidates since they carried a minimum of 1 seedlessness-related allele, and the 124 F1 hybrids were removed from the seedless grapevine breeding studies. A total of 13 individuals that carried alleles for the 3 markers associated with seedlessness were selected as genetic resources for future studies on seedless grapevine breeding.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/17473</dc:identifier>
  <dc:identifier>oai:zenodo.org:17473</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>TURKISH JOURNAL OF BIOLOGY 37(1) 101-105</dc:source>
  <dc:title>Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg x Sultani progeny</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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