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Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg x Sultani progeny

Akkurt, Murat; Cakir, Atilla; Shidfar, Mina; Mutaf, Filiz; Soylemezoglu, Gokhan


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{
  "@context": "https://schema.org/", 
  "@id": 17473, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Ankara Univ, Fac Agr, Dept Hort, TR-06100 Ankara, Turkey", 
      "name": "Akkurt, Murat"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ankara Univ, Fac Agr, Dept Hort, TR-06100 Ankara, Turkey", 
      "name": "Cakir, Atilla"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ankara Univ, Fac Agr, Dept Hort, TR-06100 Ankara, Turkey", 
      "name": "Shidfar, Mina"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ankara Univ, Inst Biotechnol, TR-06100 Ankara, Turkey", 
      "name": "Mutaf, Filiz"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ankara Univ, Fac Agr, Dept Hort, TR-06100 Ankara, Turkey", 
      "name": "Soylemezoglu, Gokhan"
    }
  ], 
  "datePublished": "2013-01-01", 
  "description": "The seedlessness-related molecular markers VMC7f2, SCC8, and SCF27 were used for the early selection of seedless individuals in the Muscat of Hamburg (seeded) x Sultani (seedless) progeny of 314 F1 hybrids. The DNA from F1 hybrids was amplified by polymerase chain reaction (PCR) using 3 markers. After digestion of the SCC8 marker PCR amplification products by restriction enzyme Bgl II, 72 individuals showed homozygous SCC8+/SCC8+ alleles at the seed development inhibitor locus, 76 individuals of the progeny amplified with the SCF27 marker produced 2.0-kb bands, and 118 individuals with seedlessness-related 198-bp alleles amplified with the VMC7f2 marker were selected as seedless grapevine candidates. Based on marker-assisted selection, 190 F1 hybrids were designated as seedless grapevine candidates since they carried a minimum of 1 seedlessness-related allele, and the 124 F1 hybrids were removed from the seedless grapevine breeding studies. A total of 13 individuals that carried alleles for the 3 markers associated with seedlessness were selected as genetic resources for future studies on seedless grapevine breeding.", 
  "headline": "Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg x Sultani progeny", 
  "identifier": 17473, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg x Sultani progeny", 
  "url": "https://aperta.ulakbim.gov.tr/record/17473"
}
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