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Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg x Sultani progeny

Akkurt, Murat; Cakir, Atilla; Shidfar, Mina; Mutaf, Filiz; Soylemezoglu, Gokhan


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        "affiliation": "Ankara Univ, Fac Agr, Dept Hort, TR-06100 Ankara, Turkey", 
        "name": "Akkurt, Murat"
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      {
        "affiliation": "Ankara Univ, Fac Agr, Dept Hort, TR-06100 Ankara, Turkey", 
        "name": "Cakir, Atilla"
      }, 
      {
        "affiliation": "Ankara Univ, Fac Agr, Dept Hort, TR-06100 Ankara, Turkey", 
        "name": "Shidfar, Mina"
      }, 
      {
        "affiliation": "Ankara Univ, Inst Biotechnol, TR-06100 Ankara, Turkey", 
        "name": "Mutaf, Filiz"
      }, 
      {
        "affiliation": "Ankara Univ, Fac Agr, Dept Hort, TR-06100 Ankara, Turkey", 
        "name": "Soylemezoglu, Gokhan"
      }
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    "description": "The seedlessness-related molecular markers VMC7f2, SCC8, and SCF27 were used for the early selection of seedless individuals in the Muscat of Hamburg (seeded) x Sultani (seedless) progeny of 314 F1 hybrids. The DNA from F1 hybrids was amplified by polymerase chain reaction (PCR) using 3 markers. After digestion of the SCC8 marker PCR amplification products by restriction enzyme Bgl II, 72 individuals showed homozygous SCC8+/SCC8+ alleles at the seed development inhibitor locus, 76 individuals of the progeny amplified with the SCF27 marker produced 2.0-kb bands, and 118 individuals with seedlessness-related 198-bp alleles amplified with the VMC7f2 marker were selected as seedless grapevine candidates. Based on marker-assisted selection, 190 F1 hybrids were designated as seedless grapevine candidates since they carried a minimum of 1 seedlessness-related allele, and the 124 F1 hybrids were removed from the seedless grapevine breeding studies. A total of 13 individuals that carried alleles for the 3 markers associated with seedlessness were selected as genetic resources for future studies on seedless grapevine breeding.", 
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    "title": "Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg x Sultani progeny"
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