1 Ocak 2013 Dergi makalesi Açık Erişim

Akkurt, Murat; Cakir, Atilla; Shidfar, Mina; Mutaf, Filiz; Soylemezoglu, Gokhan

Citation Style Language JSON

{
  "DOI": "10.3906/biy-1206-31", 
  "abstract": "The seedlessness-related molecular markers VMC7f2, SCC8, and SCF27 were used for the early selection of seedless individuals in the Muscat of Hamburg (seeded) x Sultani (seedless) progeny of 314 F1 hybrids. The DNA from F1 hybrids was amplified by polymerase chain reaction (PCR) using 3 markers. After digestion of the SCC8 marker PCR amplification products by restriction enzyme Bgl II, 72 individuals showed homozygous SCC8+/SCC8+ alleles at the seed development inhibitor locus, 76 individuals of the progeny amplified with the SCF27 marker produced 2.0-kb bands, and 118 individuals with seedlessness-related 198-bp alleles amplified with the VMC7f2 marker were selected as seedless grapevine candidates. Based on marker-assisted selection, 190 F1 hybrids were designated as seedless grapevine candidates since they carried a minimum of 1 seedlessness-related allele, and the 124 F1 hybrids were removed from the seedless grapevine breeding studies. A total of 13 individuals that carried alleles for the 3 markers associated with seedlessness were selected as genetic resources for future studies on seedless grapevine breeding.", 
  "author": [
    {
      "family": "Akkurt", 
      "given": " Murat"
    }, 
    {
      "family": "Cakir", 
      "given": " Atilla"
    }, 
    {
      "family": "Shidfar", 
      "given": " Mina"
    }, 
    {
      "family": "Mutaf", 
      "given": " Filiz"
    }, 
    {
      "family": "Soylemezoglu", 
      "given": " Gokhan"
    }
  ], 
  "container_title": "TURKISH JOURNAL OF BIOLOGY", 
  "id": "17473", 
  "issue": "1", 
  "issued": {
    "date-parts": [
      [
        2013, 
        1, 
        1
      ]
    ]
  }, 
  "page": "101-105", 
  "title": "Using seedlessness-related molecular markers in grapevine breeding for seedlessness via marker-assisted selection into Muscat of Hamburg x Sultani progeny", 
  "type": "article-journal", 
  "volume": "37"
}