1 Ocak 1996 Dergi makalesi Açık Erişim

Basalp, A; Bermek, E; Cirakoglu, B; Coka, V; Mustafaev, MI; Sarac, AS

Dublin Core

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  <dc:creator>Basalp, A</dc:creator>
  <dc:creator>Bermek, E</dc:creator>
  <dc:creator>Cirakoglu, B</dc:creator>
  <dc:creator>Coka, V</dc:creator>
  <dc:creator>Mustafaev, MI</dc:creator>
  <dc:creator>Sarac, AS</dc:creator>
  <dc:date>1996-01-01</dc:date>
  <dc:description>Complex formation between synthetic polyelectrolytes (PE) [poly-4-vinyl-N-ethyl (cetyl), pyridine bromides-PVP(R(2),R(16))], bovine serum albumin (BSA), or 17 beta-estradiol-BSA conjugate (BSA.E) was studied in neutral water, Weakly water-soluble (colloidal) complex was formed upon addition of BSA.E to PVP (R(2),R(16)) solution at pH 7, A nonrandom distribution of the protein molecules between the coils of polycations and self-assembly in the nonstochiometric polycomplex particles took place, The immunogenic properties of PVP (R(2),R(16))-BSA.E polycomplex were investigated and the specificities of produced antibodies analyzed, 17 beta-Estradiol introduced in polyelectrolyte complexes (PE-BSA) was found to invoke considerable increases in the steroid-specific immunoresponse, However, a comparative study of immunogenic activity of polycomplexes versus BSA.E + incomplete Freund's adjuvant (IFA) mixtures revealed some differences in regards to the specificity of antibody production, In contrast to IFA + BSA.E systems, polycomplexes were able to generate estradiol-as well as BSA-specific antibodies, Such a carrier-directed response may be determined by increase in inmunogenicity of weak antigenic determinants and/or by the exposure of internally located determinants upon complex formation with polyelectrolytes, Fusions following the two different immunization procedures resulted in the growth of comparable numbers of estradiol-specific monoclonal antibodies with apparently similar antigen affinities, Thus, immunizations using antigens in PEC appear to provide an efficient alternative to IFA.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/104103</dc:identifier>
  <dc:identifier>oai:zenodo.org:104103</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>HYBRIDOMA 15(3) 233-238</dc:source>
  <dc:title>Immune response to 17 beta-estradiol in polyelectrolyte complex: Antigen specificity and affinity of hybridoma clones</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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