1 Ocak 1996 Dergi makalesi Açık Erişim

Basalp, A; Bermek, E; Cirakoglu, B; Coka, V; Mustafaev, MI; Sarac, AS

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/104103</identifier>
  <creators>
    <creator>
      <creatorName>Basalp, A</creatorName>
      <givenName>A</givenName>
      <familyName>Basalp</familyName>
    </creator>
    <creator>
      <creatorName>Bermek, E</creatorName>
      <givenName>E</givenName>
      <familyName>Bermek</familyName>
    </creator>
    <creator>
      <creatorName>Cirakoglu, B</creatorName>
      <givenName>B</givenName>
      <familyName>Cirakoglu</familyName>
    </creator>
    <creator>
      <creatorName>Coka, V</creatorName>
      <givenName>V</givenName>
      <familyName>Coka</familyName>
    </creator>
    <creator>
      <creatorName>Mustafaev, MI</creatorName>
      <givenName>MI</givenName>
      <familyName>Mustafaev</familyName>
    </creator>
    <creator>
      <creatorName>Sarac, AS</creatorName>
      <givenName>AS</givenName>
      <familyName>Sarac</familyName>
    </creator>
  </creators>
  <titles>
    <title>Immune Response To 17 Beta-Estradiol In Polyelectrolyte Complex: Antigen Specificity And Affinity Of Hybridoma Clones</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>1996</publicationYear>
  <dates>
    <date dateType="Issued">1996-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/104103</alternateIdentifier>
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    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.81043/aperta.104102</relatedIdentifier>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.81043/aperta.104103</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Complex formation between synthetic polyelectrolytes (PE) [poly-4-vinyl-N-ethyl (cetyl), pyridine bromides-PVP(R(2),R(16))], bovine serum albumin (BSA), or 17 beta-estradiol-BSA conjugate (BSA.E) was studied in neutral water, Weakly water-soluble (colloidal) complex was formed upon addition of BSA.E to PVP (R(2),R(16)) solution at pH 7, A nonrandom distribution of the protein molecules between the coils of polycations and self-assembly in the nonstochiometric polycomplex particles took place, The immunogenic properties of PVP (R(2),R(16))-BSA.E polycomplex were investigated and the specificities of produced antibodies analyzed, 17 beta-Estradiol introduced in polyelectrolyte complexes (PE-BSA) was found to invoke considerable increases in the steroid-specific immunoresponse, However, a comparative study of immunogenic activity of polycomplexes versus BSA.E + incomplete Freund's adjuvant (IFA) mixtures revealed some differences in regards to the specificity of antibody production, In contrast to IFA + BSA.E systems, polycomplexes were able to generate estradiol-as well as BSA-specific antibodies, Such a carrier-directed response may be determined by increase in inmunogenicity of weak antigenic determinants and/or by the exposure of internally located determinants upon complex formation with polyelectrolytes, Fusions following the two different immunization procedures resulted in the growth of comparable numbers of estradiol-specific monoclonal antibodies with apparently similar antigen affinities, Thus, immunizations using antigens in PEC appear to provide an efficient alternative to IFA.</description>
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