1 Ocak 1996 Dergi makalesi Açık Erişim

Basalp, A; Bermek, E; Cirakoglu, B; Coka, V; Mustafaev, MI; Sarac, AS

Citation Style Language JSON

{
  "URL": "https://aperta.ulakbim.gov.tr/record/104103", 
  "abstract": "Complex formation between synthetic polyelectrolytes (PE) [poly-4-vinyl-N-ethyl (cetyl), pyridine bromides-PVP(R(2),R(16))], bovine serum albumin (BSA), or 17 beta-estradiol-BSA conjugate (BSA.E) was studied in neutral water, Weakly water-soluble (colloidal) complex was formed upon addition of BSA.E to PVP (R(2),R(16)) solution at pH 7, A nonrandom distribution of the protein molecules between the coils of polycations and self-assembly in the nonstochiometric polycomplex particles took place, The immunogenic properties of PVP (R(2),R(16))-BSA.E polycomplex were investigated and the specificities of produced antibodies analyzed, 17 beta-Estradiol introduced in polyelectrolyte complexes (PE-BSA) was found to invoke considerable increases in the steroid-specific immunoresponse, However, a comparative study of immunogenic activity of polycomplexes versus BSA.E + incomplete Freund's adjuvant (IFA) mixtures revealed some differences in regards to the specificity of antibody production, In contrast to IFA + BSA.E systems, polycomplexes were able to generate estradiol-as well as BSA-specific antibodies, Such a carrier-directed response may be determined by increase in inmunogenicity of weak antigenic determinants and/or by the exposure of internally located determinants upon complex formation with polyelectrolytes, Fusions following the two different immunization procedures resulted in the growth of comparable numbers of estradiol-specific monoclonal antibodies with apparently similar antigen affinities, Thus, immunizations using antigens in PEC appear to provide an efficient alternative to IFA.", 
  "author": [
    {
      "family": "Basalp", 
      "given": " A"
    }, 
    {
      "family": "Bermek", 
      "given": " E"
    }, 
    {
      "family": "Cirakoglu", 
      "given": " B"
    }, 
    {
      "family": "Coka", 
      "given": " V"
    }, 
    {
      "family": "Mustafaev", 
      "given": " MI"
    }, 
    {
      "family": "Sarac", 
      "given": " AS"
    }
  ], 
  "container_title": "HYBRIDOMA", 
  "id": "104103", 
  "issue": "3", 
  "issued": {
    "date-parts": [
      [
        1996, 
        1, 
        1
      ]
    ]
  }, 
  "page": "233-238", 
  "title": "Immune response to 17 beta-estradiol in polyelectrolyte complex: Antigen specificity and affinity of hybridoma clones", 
  "type": "article-journal", 
  "volume": "15"
}