1 Ocak 1996 Dergi makalesi Açık Erişim

Basalp, A; Bermek, E; Cirakoglu, B; Coka, V; Mustafaev, MI; Sarac, AS

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{
  "@context": "https://schema.org/", 
  "@id": 104103, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "name": "Basalp, A"
    }, 
    {
      "@type": "Person", 
      "name": "Bermek, E"
    }, 
    {
      "@type": "Person", 
      "name": "Cirakoglu, B"
    }, 
    {
      "@type": "Person", 
      "name": "Coka, V"
    }, 
    {
      "@type": "Person", 
      "name": "Mustafaev, MI"
    }, 
    {
      "@type": "Person", 
      "name": "Sarac, AS"
    }
  ], 
  "datePublished": "1996-01-01", 
  "description": "Complex formation between synthetic polyelectrolytes (PE) [poly-4-vinyl-N-ethyl (cetyl), pyridine bromides-PVP(R(2),R(16))], bovine serum albumin (BSA), or 17 beta-estradiol-BSA conjugate (BSA.E) was studied in neutral water, Weakly water-soluble (colloidal) complex was formed upon addition of BSA.E to PVP (R(2),R(16)) solution at pH 7, A nonrandom distribution of the protein molecules between the coils of polycations and self-assembly in the nonstochiometric polycomplex particles took place, The immunogenic properties of PVP (R(2),R(16))-BSA.E polycomplex were investigated and the specificities of produced antibodies analyzed, 17 beta-Estradiol introduced in polyelectrolyte complexes (PE-BSA) was found to invoke considerable increases in the steroid-specific immunoresponse, However, a comparative study of immunogenic activity of polycomplexes versus BSA.E + incomplete Freund's adjuvant (IFA) mixtures revealed some differences in regards to the specificity of antibody production, In contrast to IFA + BSA.E systems, polycomplexes were able to generate estradiol-as well as BSA-specific antibodies, Such a carrier-directed response may be determined by increase in inmunogenicity of weak antigenic determinants and/or by the exposure of internally located determinants upon complex formation with polyelectrolytes, Fusions following the two different immunization procedures resulted in the growth of comparable numbers of estradiol-specific monoclonal antibodies with apparently similar antigen affinities, Thus, immunizations using antigens in PEC appear to provide an efficient alternative to IFA.", 
  "headline": "Immune response to 17 beta-estradiol in polyelectrolyte complex: Antigen specificity and affinity of hybridoma clones", 
  "identifier": 104103, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Immune response to 17 beta-estradiol in polyelectrolyte complex: Antigen specificity and affinity of hybridoma clones", 
  "url": "https://aperta.ulakbim.gov.tr/record/104103"
}