1 Ocak 1996 Dergi makalesi Açık Erişim

Gumusel, F; Kiremit, N; Issever, S; Ogras, TT; Bermek, E

Dublin Core

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  <dc:creator>Gumusel, F</dc:creator>
  <dc:creator>Kiremit, N</dc:creator>
  <dc:creator>Issever, S</dc:creator>
  <dc:creator>Ogras, TT</dc:creator>
  <dc:creator>Bermek, E</dc:creator>
  <dc:date>1996-01-01</dc:date>
  <dc:description>The penicillin acylase gene (pac) amplified by polymerase chain reaction (PCR) from Escherichia coli (E. coli) ATCC11105 genomic DNA was cloned into pUC9, pEMBL9(+) and pCP40 vectors. A penicillin acylase precursor was overexpressed at 26 degrees C from pac-pEMBL9(+) and pac-pCP40 constructs transformed into E.coli strains JM109 and pCI857 containing HB101, respectively. With the thermo-inducible pac-pCP40 construct some level of mature alpha and beta subunits and varying degrees of enzyme activity were also detected.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/103387</dc:identifier>
  <dc:identifier>oai:zenodo.org:103387</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL 38(2) 315-324</dc:source>
  <dc:title>PCR-cloning of the pac gene from E-coli ATCC11105 - Observations on the expression of a recombinant periplasmic protein</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
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