1 Ocak 1996 Dergi makalesi Açık Erişim

Gumusel, F; Kiremit, N; Issever, S; Ogras, TT; Bermek, E

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  "@id": 103387, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "name": "Gumusel, F"
    }, 
    {
      "@type": "Person", 
      "name": "Kiremit, N"
    }, 
    {
      "@type": "Person", 
      "name": "Issever, S"
    }, 
    {
      "@type": "Person", 
      "name": "Ogras, TT"
    }, 
    {
      "@type": "Person", 
      "name": "Bermek, E"
    }
  ], 
  "datePublished": "1996-01-01", 
  "description": "The penicillin acylase gene (pac) amplified by polymerase chain reaction (PCR) from Escherichia coli (E. coli) ATCC11105 genomic DNA was cloned into pUC9, pEMBL9(+) and pCP40 vectors. A penicillin acylase precursor was overexpressed at 26 degrees C from pac-pEMBL9(+) and pac-pCP40 constructs transformed into E.coli strains JM109 and pCI857 containing HB101, respectively. With the thermo-inducible pac-pCP40 construct some level of mature alpha and beta subunits and varying degrees of enzyme activity were also detected.", 
  "headline": "PCR-cloning of the pac gene from E-coli ATCC11105 - Observations on the expression of a recombinant periplasmic protein", 
  "identifier": 103387, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "PCR-cloning of the pac gene from E-coli ATCC11105 - Observations on the expression of a recombinant periplasmic protein", 
  "url": "https://aperta.ulakbim.gov.tr/record/103387"
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