1 Ocak 1996 Dergi makalesi Açık Erişim

Gumusel, F; Kiremit, N; Issever, S; Ogras, TT; Bermek, E

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/103387</identifier>
  <creators>
    <creator>
      <creatorName>Gumusel, F</creatorName>
      <givenName>F</givenName>
      <familyName>Gumusel</familyName>
    </creator>
    <creator>
      <creatorName>Kiremit, N</creatorName>
      <givenName>N</givenName>
      <familyName>Kiremit</familyName>
    </creator>
    <creator>
      <creatorName>Issever, S</creatorName>
      <givenName>S</givenName>
      <familyName>Issever</familyName>
    </creator>
    <creator>
      <creatorName>Ogras, TT</creatorName>
      <givenName>TT</givenName>
      <familyName>Ogras</familyName>
    </creator>
    <creator>
      <creatorName>Bermek, E</creatorName>
      <givenName>E</givenName>
      <familyName>Bermek</familyName>
    </creator>
  </creators>
  <titles>
    <title>Pcr-Cloning Of The Pac Gene From E-Coli Atcc11105 - Observations On The Expression Of A Recombinant Periplasmic Protein</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>1996</publicationYear>
  <dates>
    <date dateType="Issued">1996-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/103387</alternateIdentifier>
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    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsVersionOf">10.81043/aperta.103386</relatedIdentifier>
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    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">The penicillin acylase gene (pac) amplified by polymerase chain reaction (PCR) from Escherichia coli (E. coli) ATCC11105 genomic DNA was cloned into pUC9, pEMBL9(+) and pCP40 vectors. A penicillin acylase precursor was overexpressed at 26 degrees C from pac-pEMBL9(+) and pac-pCP40 constructs transformed into E.coli strains JM109 and pCI857 containing HB101, respectively. With the thermo-inducible pac-pCP40 construct some level of mature alpha and beta subunits and varying degrees of enzyme activity were also detected.</description>
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