1 Ocak 1996 Dergi makalesi Açık Erişim

Gumusel, F; Kiremit, N; Issever, S; Ogras, TT; Bermek, E

Citation Style Language JSON

{
  "URL": "https://aperta.ulakbim.gov.tr/record/103387", 
  "abstract": "The penicillin acylase gene (pac) amplified by polymerase chain reaction (PCR) from Escherichia coli (E. coli) ATCC11105 genomic DNA was cloned into pUC9, pEMBL9(+) and pCP40 vectors. A penicillin acylase precursor was overexpressed at 26 degrees C from pac-pEMBL9(+) and pac-pCP40 constructs transformed into E.coli strains JM109 and pCI857 containing HB101, respectively. With the thermo-inducible pac-pCP40 construct some level of mature alpha and beta subunits and varying degrees of enzyme activity were also detected.", 
  "author": [
    {
      "family": "Gumusel", 
      "given": " F"
    }, 
    {
      "family": "Kiremit", 
      "given": " N"
    }, 
    {
      "family": "Issever", 
      "given": " S"
    }, 
    {
      "family": "Ogras", 
      "given": " TT"
    }, 
    {
      "family": "Bermek", 
      "given": " E"
    }
  ], 
  "container_title": "BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL", 
  "id": "103387", 
  "issue": "2", 
  "issued": {
    "date-parts": [
      [
        1996, 
        1, 
        1
      ]
    ]
  }, 
  "page": "315-324", 
  "title": "PCR-cloning of the pac gene from E-coli ATCC11105 - Observations on the expression of a recombinant periplasmic protein", 
  "type": "article-journal", 
  "volume": "38"
}