1 Ocak 2020 Dergi makalesi Açık Erişim

Subasi, Elif; Atalay, Esra Bulut; Erdogan, Duygu; Sen, Betill; Pakyapan, Bilge; Kayali, Hulya Ayar

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  "@context": "https://schema.org/", 
  "@id": 9615, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Dokuz Eylul Univ, Fac Sci, Dept Chem, TR-35160 Izmir, Turkey", 
      "name": "Subasi, Elif"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Dokuz Eylul Univ, Izmir Int Biomed & Genome Inst, TR-35340 Izmir, Turkey", 
      "name": "Atalay, Esra Bulut"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Dokuz Eylul Univ, Izmir Int Biomed & Genome Inst, TR-35340 Izmir, Turkey", 
      "name": "Erdogan, Duygu"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Dokuz Eylul Univ, Fac Sci, Dept Phys, TR-35160 Izmir, Turkey", 
      "name": "Sen, Betill"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Ege Univ, Inst Sci & Technol, TR-35100 Izmir, Turkey", 
      "name": "Pakyapan, Bilge"
    }, 
    {
      "@type": "Person", 
      "name": "Kayali, Hulya Ayar"
    }
  ], 
  "datePublished": "2020-01-01", 
  "description": "In the current study, organoruthenium(II)-arene complexes (I-IV) have been prepared by the reaction of ({(eta(6)-pcym)RuCl}(2)(mu-Cl)(2)] with new thiosemicarbazones (TSC1-4).The isolate was analyzed using elemental analysis, FT-IR, H-1 and C-13 NMR spectroscopy and single-crystal XRD. Subsequently, the complexes and TSC ligands were assessed for anticancer properties in vitro against three different colorectal cancer stage's cell lines (Caco-2, DLD-1, and SW620) and a noncancerous cell line (CCD18Co). The complexes (I-IV) showed higher cytotoxicity with low IC50 values as 0.1-0.33 mu M in colorectal cell lines except for SW620 (47.4-84.20 mu M) than in a noncancerous cell. Complex I was 2.8 and 24.5-fold more active against Caco-2 and DLD-1 than CCD18Co, respectively. The complexes (I-IV) accumulated at a high concentration in the cell nuclei and caused cell cycle arrest by affecting the G0/G1 and/or G2/M phase and showed high binding affinity with CT-DNA (Kb = 10(4) M-1). The expression of Caspase-3 and Caspase-9 apoptosis-related protein levels was slightly upregulated and Atg5 autophagy-related protein level was clearly downregulated according to control and 5-FU-treated cells after complex I and II treatment. Furthermore, it was observed that cytotoxicity of the complexes is decreased while cancer progresses. Altogether, this study indicates that all organoruthenium (II)-arene complexes (particularly complex I) can be a promising alternative to platinum counterparts in cancer treatment.", 
  "headline": "Synthesis and characterization of thiosemicarbazone-functionalized organoruthenium (II)-arene complexes: Investigation of antitumor characteristics in colorectal cancer cell lines", 
  "identifier": 9615, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Synthesis and characterization of thiosemicarbazone-functionalized organoruthenium (II)-arene complexes: Investigation of antitumor characteristics in colorectal cancer cell lines", 
  "url": "https://aperta.ulakbim.gov.tr/record/9615"
}