1 Ocak 2006 Dergi makalesi Açık Erişim

Altinkut, Ahu; Raskina, Olga; Nevo, Eviatar; Belyayev, Alexander

Dublin Core

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<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd">
  <dc:creator>Altinkut, Ahu</dc:creator>
  <dc:creator>Raskina, Olga</dc:creator>
  <dc:creator>Nevo, Eviatar</dc:creator>
  <dc:creator>Belyayev, Alexander</dc:creator>
  <dc:date>2006-01-01</dc:date>
  <dc:description>Belonging to Class II of transposable elements, En/Spm transposons are widespread in a variety of distantly related plant species. Here, we report on the sequence conservation of the transposase region from sequence analyses of En/Spm-like transposons from Poaceae species, namely Zingeria biebersteiniana, Zingeria trichopoda, Triticum monococcum, Triticum urartu, Hordeum spontaneum, and Aegilops speltoides. The transposase region of En/Spm-like transposons was cloned, sequenced, and compared with equivalent regions of Oryza and Arabidopsis from the gene bank database. Southern blot analysis indicated that the En/Spm transposon was present in low (Hordeum spontaneum, Triticum monococcum, Triticum urartu) through medium (Zingeria bieberstiana, Zingeria trichopoda) to relatively high (Aegilops speltoides) copy numbers in Poaceae species. A cytogenetic analysis of the chromosomal distribution of En/Spm transposons revealed the concurence of the chromosomal localization of the En/Spm clusters with mobile clusters of rDNA. An analysis of En/Spm-like transposase amino acid sequences was carried out to investigate sequence divergence between 5 genera - Triticum, Aegilops, Zingeria, Oryza and Arabidopsis. A distance matrix.-was generated; apparently, En/Spm-like transposase sequences shared the highest sequence homology intra-generically and, as expected, these sequences were significantly diverged from those of O. sativa and A. thaliana. A sequence comparison of En/Spin-like transposase coding regions defined that the intra-genomic complex of En/Spin-like transposons could be viewed as relatively independent, vertically transmitted, and permanently active systems inside higher plant genomes. The sequence data from this article was deposited in the EMBL/GenBank Data Libraries under the accession nos. AY707995 - AY707996 - AY707997 - AY707998 - AY707999 - AY708000 - AY708001 - AY708002 - AY708003 AY708004 - AY708005 - AY708005 - AY265312.</dc:description>
  <dc:identifier>https://aperta.ulakbim.gov.trrecord/96071</dc:identifier>
  <dc:identifier>oai:zenodo.org:96071</dc:identifier>
  <dc:rights>info:eu-repo/semantics/openAccess</dc:rights>
  <dc:rights>http://www.opendefinition.org/licenses/cc-by</dc:rights>
  <dc:source>CELLULAR &amp; MOLECULAR BIOLOGY LETTERS 11(2) 214-229</dc:source>
  <dc:title>En/spm-like transposons in Poaceae species: Transposase sequence variability and chromosomal distribution</dc:title>
  <dc:type>info:eu-repo/semantics/article</dc:type>
  <dc:type>publication-article</dc:type>
</oai_dc:dc>