1 Ocak 2006 Dergi makalesi Açık Erişim

Altinkut, Ahu; Raskina, Olga; Nevo, Eviatar; Belyayev, Alexander

JSON

{
  "conceptrecid": "96070", 
  "created": "2021-03-16T13:24:28.026482+00:00", 
  "doi": "10.2478/s11658-006-0017-3", 
  "files": [
    {
      "bucket": "ff69e284-a433-45df-8533-a1c03c2817e3", 
      "checksum": "md5:45ee2002015e7d57dd65f632729596a5", 
      "key": "bib-4a46861d-acb0-4145-9588-58f3e3b357b2.txt", 
      "links": {
        "self": "https://aperta.ulakbim.gov.tr/api/files/ff69e284-a433-45df-8533-a1c03c2817e3/bib-4a46861d-acb0-4145-9588-58f3e3b357b2.txt"
      }, 
      "size": 219, 
      "type": "txt"
    }
  ], 
  "id": 96071, 
  "links": {
    "badge": "https://aperta.ulakbim.gov.tr/badge/doi/10.2478/s11658-006-0017-3.svg", 
    "bucket": "https://aperta.ulakbim.gov.tr/api/files/ff69e284-a433-45df-8533-a1c03c2817e3", 
    "doi": "https://doi.org/10.2478/s11658-006-0017-3", 
    "html": "https://aperta.ulakbim.gov.tr/record/96071", 
    "latest": "https://aperta.ulakbim.gov.tr/api/records/96071", 
    "latest_html": "https://aperta.ulakbim.gov.tr/record/96071"
  }, 
  "metadata": {
    "access_right": "open", 
    "access_right_category": "success", 
    "communities": [
      {
        "id": "tubitak-adresli-yayinlar"
      }
    ], 
    "creators": [
      {
        "name": "Altinkut, Ahu"
      }, 
      {
        "name": "Raskina, Olga"
      }, 
      {
        "name": "Nevo, Eviatar"
      }, 
      {
        "name": "Belyayev, Alexander"
      }
    ], 
    "description": "Belonging to Class II of transposable elements, En/Spm transposons are widespread in a variety of distantly related plant species. Here, we report on the sequence conservation of the transposase region from sequence analyses of En/Spm-like transposons from Poaceae species, namely Zingeria biebersteiniana, Zingeria trichopoda, Triticum monococcum, Triticum urartu, Hordeum spontaneum, and Aegilops speltoides. The transposase region of En/Spm-like transposons was cloned, sequenced, and compared with equivalent regions of Oryza and Arabidopsis from the gene bank database. Southern blot analysis indicated that the En/Spm transposon was present in low (Hordeum spontaneum, Triticum monococcum, Triticum urartu) through medium (Zingeria bieberstiana, Zingeria trichopoda) to relatively high (Aegilops speltoides) copy numbers in Poaceae species. A cytogenetic analysis of the chromosomal distribution of En/Spm transposons revealed the concurence of the chromosomal localization of the En/Spm clusters with mobile clusters of rDNA. An analysis of En/Spm-like transposase amino acid sequences was carried out to investigate sequence divergence between 5 genera - Triticum, Aegilops, Zingeria, Oryza and Arabidopsis. A distance matrix.-was generated; apparently, En/Spm-like transposase sequences shared the highest sequence homology intra-generically and, as expected, these sequences were significantly diverged from those of O. sativa and A. thaliana. A sequence comparison of En/Spin-like transposase coding regions defined that the intra-genomic complex of En/Spin-like transposons could be viewed as relatively independent, vertically transmitted, and permanently active systems inside higher plant genomes. The sequence data from this article was deposited in the EMBL/GenBank Data Libraries under the accession nos. AY707995 - AY707996 - AY707997 - AY707998 - AY707999 - AY708000 - AY708001 - AY708002 - AY708003 AY708004 - AY708005 - AY708005 - AY265312.", 
    "doi": "10.2478/s11658-006-0017-3", 
    "has_grant": false, 
    "journal": {
      "issue": "2", 
      "pages": "214-229", 
      "title": "CELLULAR & MOLECULAR BIOLOGY LETTERS", 
      "volume": "11"
    }, 
    "license": {
      "id": "cc-by"
    }, 
    "publication_date": "2006-01-01", 
    "relations": {
      "version": [
        {
          "count": 1, 
          "index": 0, 
          "is_last": true, 
          "last_child": {
            "pid_type": "recid", 
            "pid_value": "96071"
          }, 
          "parent": {
            "pid_type": "recid", 
            "pid_value": "96070"
          }
        }
      ]
    }, 
    "resource_type": {
      "subtype": "article", 
      "title": "Dergi makalesi", 
      "type": "publication"
    }, 
    "title": "En/spm-like transposons in Poaceae species: Transposase sequence variability and chromosomal distribution"
  }, 
  "owners": [
    1
  ], 
  "revision": 1, 
  "stats": {
    "downloads": 8.0, 
    "unique_downloads": 7.0, 
    "unique_views": 23.0, 
    "version_downloads": 8.0, 
    "version_unique_downloads": 7.0, 
    "version_unique_views": 23.0, 
    "version_views": 24.0, 
    "version_volume": 1752.0, 
    "views": 24.0, 
    "volume": 1752.0
  }, 
  "updated": "2021-03-16T13:24:28.090951+00:00"
}