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Altinkut, Ahu; Raskina, Olga; Nevo, Eviatar; Belyayev, Alexander
<?xml version='1.0' encoding='utf-8'?> <resource xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://datacite.org/schema/kernel-4" xsi:schemaLocation="http://datacite.org/schema/kernel-4 http://schema.datacite.org/meta/kernel-4.1/metadata.xsd"> <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/96071</identifier> <creators> <creator> <creatorName>Altinkut, Ahu</creatorName> <givenName>Ahu</givenName> <familyName>Altinkut</familyName> </creator> <creator> <creatorName>Raskina, Olga</creatorName> <givenName>Olga</givenName> <familyName>Raskina</familyName> </creator> <creator> <creatorName>Nevo, Eviatar</creatorName> <givenName>Eviatar</givenName> <familyName>Nevo</familyName> </creator> <creator> <creatorName>Belyayev, Alexander</creatorName> <givenName>Alexander</givenName> <familyName>Belyayev</familyName> </creator> </creators> <titles> <title>En/Spm-Like Transposons In Poaceae Species: Transposase Sequence Variability And Chromosomal Distribution</title> </titles> <publisher>Aperta</publisher> <publicationYear>2006</publicationYear> <dates> <date dateType="Issued">2006-01-01</date> </dates> <resourceType resourceTypeGeneral="Text">Journal article</resourceType> <alternateIdentifiers> <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/96071</alternateIdentifier> </alternateIdentifiers> <relatedIdentifiers> <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.2478/s11658-006-0017-3</relatedIdentifier> </relatedIdentifiers> <rightsList> <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights> <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights> </rightsList> <descriptions> <description descriptionType="Abstract">Belonging to Class II of transposable elements, En/Spm transposons are widespread in a variety of distantly related plant species. Here, we report on the sequence conservation of the transposase region from sequence analyses of En/Spm-like transposons from Poaceae species, namely Zingeria biebersteiniana, Zingeria trichopoda, Triticum monococcum, Triticum urartu, Hordeum spontaneum, and Aegilops speltoides. The transposase region of En/Spm-like transposons was cloned, sequenced, and compared with equivalent regions of Oryza and Arabidopsis from the gene bank database. Southern blot analysis indicated that the En/Spm transposon was present in low (Hordeum spontaneum, Triticum monococcum, Triticum urartu) through medium (Zingeria bieberstiana, Zingeria trichopoda) to relatively high (Aegilops speltoides) copy numbers in Poaceae species. A cytogenetic analysis of the chromosomal distribution of En/Spm transposons revealed the concurence of the chromosomal localization of the En/Spm clusters with mobile clusters of rDNA. An analysis of En/Spm-like transposase amino acid sequences was carried out to investigate sequence divergence between 5 genera - Triticum, Aegilops, Zingeria, Oryza and Arabidopsis. A distance matrix.-was generated; apparently, En/Spm-like transposase sequences shared the highest sequence homology intra-generically and, as expected, these sequences were significantly diverged from those of O. sativa and A. thaliana. A sequence comparison of En/Spin-like transposase coding regions defined that the intra-genomic complex of En/Spin-like transposons could be viewed as relatively independent, vertically transmitted, and permanently active systems inside higher plant genomes. The sequence data from this article was deposited in the EMBL/GenBank Data Libraries under the accession nos. AY707995 - AY707996 - AY707997 - AY707998 - AY707999 - AY708000 - AY708001 - AY708002 - AY708003 AY708004 - AY708005 - AY708005 - AY265312.</description> </descriptions> </resource>
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