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En/spm-like transposons in Poaceae species: Transposase sequence variability and chromosomal distribution

Altinkut, Ahu; Raskina, Olga; Nevo, Eviatar; Belyayev, Alexander


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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/96071</identifier>
  <creators>
    <creator>
      <creatorName>Altinkut, Ahu</creatorName>
      <givenName>Ahu</givenName>
      <familyName>Altinkut</familyName>
    </creator>
    <creator>
      <creatorName>Raskina, Olga</creatorName>
      <givenName>Olga</givenName>
      <familyName>Raskina</familyName>
    </creator>
    <creator>
      <creatorName>Nevo, Eviatar</creatorName>
      <givenName>Eviatar</givenName>
      <familyName>Nevo</familyName>
    </creator>
    <creator>
      <creatorName>Belyayev, Alexander</creatorName>
      <givenName>Alexander</givenName>
      <familyName>Belyayev</familyName>
    </creator>
  </creators>
  <titles>
    <title>En/Spm-Like Transposons In Poaceae Species: Transposase Sequence Variability And Chromosomal Distribution</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2006</publicationYear>
  <dates>
    <date dateType="Issued">2006-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/96071</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.2478/s11658-006-0017-3</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Belonging to Class II of transposable elements, En/Spm transposons are widespread in a variety of distantly related plant species. Here, we report on the sequence conservation of the transposase region from sequence analyses of En/Spm-like transposons from Poaceae species, namely Zingeria biebersteiniana, Zingeria trichopoda, Triticum monococcum, Triticum urartu, Hordeum spontaneum, and Aegilops speltoides. The transposase region of En/Spm-like transposons was cloned, sequenced, and compared with equivalent regions of Oryza and Arabidopsis from the gene bank database. Southern blot analysis indicated that the En/Spm transposon was present in low (Hordeum spontaneum, Triticum monococcum, Triticum urartu) through medium (Zingeria bieberstiana, Zingeria trichopoda) to relatively high (Aegilops speltoides) copy numbers in Poaceae species. A cytogenetic analysis of the chromosomal distribution of En/Spm transposons revealed the concurence of the chromosomal localization of the En/Spm clusters with mobile clusters of rDNA. An analysis of En/Spm-like transposase amino acid sequences was carried out to investigate sequence divergence between 5 genera - Triticum, Aegilops, Zingeria, Oryza and Arabidopsis. A distance matrix.-was generated; apparently, En/Spm-like transposase sequences shared the highest sequence homology intra-generically and, as expected, these sequences were significantly diverged from those of O. sativa and A. thaliana. A sequence comparison of En/Spin-like transposase coding regions defined that the intra-genomic complex of En/Spin-like transposons could be viewed as relatively independent, vertically transmitted, and permanently active systems inside higher plant genomes. The sequence data from this article was deposited in the EMBL/GenBank Data Libraries under the accession nos. AY707995 - AY707996 - AY707997 - AY707998 - AY707999 - AY708000 - AY708001 - AY708002 - AY708003 AY708004 - AY708005 - AY708005 - AY265312.</description>
  </descriptions>
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