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Rapid and reliable 25-OH vitamin D2 and 25-OH vitamin D3 measurements by multitasker LC-MS/MS

Goren, Ahmet C.; Sahin, Gozde; Gumilcineli, Ismail; Binici, Burcu


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        "affiliation": "TUBITAK UME Natl Metrol Inst, Organ Chem Labs, TR-41470 Gebze, Turkey", 
        "name": "Goren, Ahmet C."
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        "affiliation": "Zivak Technol, Gursel Mh Eski Besiktas Cad 44-1-2, TR-34400 Istanbul, Turkey", 
        "name": "Sahin, Gozde"
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      {
        "affiliation": "Zivak Technol, Gursel Mh Eski Besiktas Cad 44-1-2, TR-34400 Istanbul, Turkey", 
        "name": "Gumilcineli, Ismail"
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      {
        "name": "Binici, Burcu"
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    "description": "The 25-OH Vitamin D2 and 25-OH Vitamin D3 measurement method was validated using a Zivak Multitasker Fully Automated Sample Preparation and Injection System coupled to a MS detector. The current method incorporates all sample preparations, SPE clean up procedures, LC and MS facilities into an all in one system. This system can report patient data within 6 minutes including the SPE and Sample preparation and mass spectrometry analysis time. The correlation coefficient of the developed method was greater than 0.99 for measurands, 25-OH-vitamin Ds, in the calibration range. The measurement uncertainty of the measurements were evaluated and found as 7.7 % and 7.6 % for 25-OH vitamin D2 and 25-OH-vitamin D3, respectively.", 
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      "pages": "17-25", 
      "title": "JOURNAL OF CHEMICAL METROLOGY", 
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