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Covalent immobilization of invertase on PAMAM-dendrimer modified superparamagnetic iron oxide nanoparticles

Uzun, K.; Cevik, E.; Senel, M.; Sozeri, H.; Baykal, A.; Abasiyanik, M. F.; Toprak, M. S.


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{
  "@context": "https://schema.org/", 
  "@id": 92915, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Fatih Univ, Fac Arts & Sci, Dept Chem, TR-34500 Istanbul, Turkey", 
      "name": "Uzun, K."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Fatih Univ, Fac Arts & Sci, Dept Chem, TR-34500 Istanbul, Turkey", 
      "name": "Cevik, E."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Fatih Univ, Fac Arts & Sci, Dept Chem, TR-34500 Istanbul, Turkey", 
      "name": "Senel, M."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Natl Metrol Inst, TUBITAK UME, TR-41470 Gebze, Turkey", 
      "name": "Sozeri, H."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Fatih Univ, Fac Arts & Sci, Dept Chem, TR-34500 Istanbul, Turkey", 
      "name": "Baykal, A."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Fatih Univ, Fac Engn, Dept Genet & Bioengn, TR-34500 Istanbul, Turkey", 
      "name": "Abasiyanik, M. F."
    }, 
    {
      "@type": "Person", 
      "affiliation": "Royal Inst Technol KTH, Dept Funct Mat, S-16440 Stockholm, Sweden", 
      "name": "Toprak, M. S."
    }
  ], 
  "datePublished": "2010-01-01", 
  "description": "In this study, polyamidoamine (PAMAM) dendrimer was synthesized on the surface of superparamagnetite nanoparticles to enhance invertase immobilization. The amount of immobilized enzyme on the surface-hyperbranched magnetite nanoparticle was up to 2.5 times (i.e., 250%) as much as that of magnetite nanoparticle modified with only amino silane. Maximum reaction rate (V (max)) and Michaelis-Menten constant (K (m)) were determined for the free and immobilized enzymes. Various characteristics of immobilized invertase such as; the temperature activity, thermal stability, operational stability, and storage stability were evaluated and results revealed that stability of the enzyme is improved upon immobilization.", 
  "headline": "Covalent immobilization of invertase on PAMAM-dendrimer modified superparamagnetic iron oxide nanoparticles", 
  "identifier": 92915, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Covalent immobilization of invertase on PAMAM-dendrimer modified superparamagnetic iron oxide nanoparticles", 
  "url": "https://aperta.ulakbim.gov.tr/record/92915"
}
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