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Covalent immobilization of invertase on PAMAM-dendrimer modified superparamagnetic iron oxide nanoparticles

Uzun, K.; Cevik, E.; Senel, M.; Sozeri, H.; Baykal, A.; Abasiyanik, M. F.; Toprak, M. S.


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{
  "DOI": "10.1007/s11051-010-9902-9", 
  "abstract": "In this study, polyamidoamine (PAMAM) dendrimer was synthesized on the surface of superparamagnetite nanoparticles to enhance invertase immobilization. The amount of immobilized enzyme on the surface-hyperbranched magnetite nanoparticle was up to 2.5 times (i.e., 250%) as much as that of magnetite nanoparticle modified with only amino silane. Maximum reaction rate (V (max)) and Michaelis-Menten constant (K (m)) were determined for the free and immobilized enzymes. Various characteristics of immobilized invertase such as; the temperature activity, thermal stability, operational stability, and storage stability were evaluated and results revealed that stability of the enzyme is improved upon immobilization.", 
  "author": [
    {
      "family": "Uzun", 
      "given": " K."
    }, 
    {
      "family": "Cevik", 
      "given": " E."
    }, 
    {
      "family": "Senel", 
      "given": " M."
    }, 
    {
      "family": "Sozeri", 
      "given": " H."
    }, 
    {
      "family": "Baykal", 
      "given": " A."
    }, 
    {
      "family": "Abasiyanik", 
      "given": " M. F."
    }, 
    {
      "family": "Toprak", 
      "given": " M. S."
    }
  ], 
  "container_title": "JOURNAL OF NANOPARTICLE RESEARCH", 
  "id": "92915", 
  "issue": "8", 
  "issued": {
    "date-parts": [
      [
        2010, 
        1, 
        1
      ]
    ]
  }, 
  "page": "3057-3067", 
  "title": "Covalent immobilization of invertase on PAMAM-dendrimer modified superparamagnetic iron oxide nanoparticles", 
  "type": "article-journal", 
  "volume": "12"
}
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