Dergi makalesi Açık Erişim
Uzun, K.; Cevik, E.; Senel, M.; Sozeri, H.; Baykal, A.; Abasiyanik, M. F.; Toprak, M. S.
{
"DOI": "10.1007/s11051-010-9902-9",
"abstract": "In this study, polyamidoamine (PAMAM) dendrimer was synthesized on the surface of superparamagnetite nanoparticles to enhance invertase immobilization. The amount of immobilized enzyme on the surface-hyperbranched magnetite nanoparticle was up to 2.5 times (i.e., 250%) as much as that of magnetite nanoparticle modified with only amino silane. Maximum reaction rate (V (max)) and Michaelis-Menten constant (K (m)) were determined for the free and immobilized enzymes. Various characteristics of immobilized invertase such as; the temperature activity, thermal stability, operational stability, and storage stability were evaluated and results revealed that stability of the enzyme is improved upon immobilization.",
"author": [
{
"family": "Uzun",
"given": " K."
},
{
"family": "Cevik",
"given": " E."
},
{
"family": "Senel",
"given": " M."
},
{
"family": "Sozeri",
"given": " H."
},
{
"family": "Baykal",
"given": " A."
},
{
"family": "Abasiyanik",
"given": " M. F."
},
{
"family": "Toprak",
"given": " M. S."
}
],
"container_title": "JOURNAL OF NANOPARTICLE RESEARCH",
"id": "92915",
"issue": "8",
"issued": {
"date-parts": [
[
2010,
1,
1
]
]
},
"page": "3057-3067",
"title": "Covalent immobilization of invertase on PAMAM-dendrimer modified superparamagnetic iron oxide nanoparticles",
"type": "article-journal",
"volume": "12"
}
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