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Covalent immobilization of invertase on PAMAM-dendrimer modified superparamagnetic iron oxide nanoparticles

Uzun, K.; Cevik, E.; Senel, M.; Sozeri, H.; Baykal, A.; Abasiyanik, M. F.; Toprak, M. S.


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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/92915</identifier>
  <creators>
    <creator>
      <creatorName>Uzun, K.</creatorName>
      <givenName>K.</givenName>
      <familyName>Uzun</familyName>
      <affiliation>Fatih Univ, Fac Arts &amp; Sci, Dept Chem, TR-34500 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Cevik, E.</creatorName>
      <givenName>E.</givenName>
      <familyName>Cevik</familyName>
      <affiliation>Fatih Univ, Fac Arts &amp; Sci, Dept Chem, TR-34500 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Senel, M.</creatorName>
      <givenName>M.</givenName>
      <familyName>Senel</familyName>
      <affiliation>Fatih Univ, Fac Arts &amp; Sci, Dept Chem, TR-34500 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Sozeri, H.</creatorName>
      <givenName>H.</givenName>
      <familyName>Sozeri</familyName>
      <affiliation>Natl Metrol Inst, TUBITAK UME, TR-41470 Gebze, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Baykal, A.</creatorName>
      <givenName>A.</givenName>
      <familyName>Baykal</familyName>
      <affiliation>Fatih Univ, Fac Arts &amp; Sci, Dept Chem, TR-34500 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Abasiyanik, M. F.</creatorName>
      <givenName>M. F.</givenName>
      <familyName>Abasiyanik</familyName>
      <affiliation>Fatih Univ, Fac Engn, Dept Genet &amp; Bioengn, TR-34500 Istanbul, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Toprak, M. S.</creatorName>
      <givenName>M. S.</givenName>
      <familyName>Toprak</familyName>
      <affiliation>Royal Inst Technol KTH, Dept Funct Mat, S-16440 Stockholm, Sweden</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Covalent Immobilization Of Invertase On Pamam-Dendrimer Modified Superparamagnetic Iron Oxide Nanoparticles</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2010</publicationYear>
  <dates>
    <date dateType="Issued">2010-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/92915</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1007/s11051-010-9902-9</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">In this study, polyamidoamine (PAMAM) dendrimer was synthesized on the surface of superparamagnetite nanoparticles to enhance invertase immobilization. The amount of immobilized enzyme on the surface-hyperbranched magnetite nanoparticle was up to 2.5 times (i.e., 250%) as much as that of magnetite nanoparticle modified with only amino silane. Maximum reaction rate (V (max)) and Michaelis-Menten constant (K (m)) were determined for the free and immobilized enzymes. Various characteristics of immobilized invertase such as; the temperature activity, thermal stability, operational stability, and storage stability were evaluated and results revealed that stability of the enzyme is improved upon immobilization.</description>
  </descriptions>
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