1 Ocak 2021 Dergi makalesi Açık Erişim

Kayihan, Doga Selin; Kayihan, Ceyhun; Ciftci, Yelda Ozden

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{
  "@context": "https://schema.org/", 
  "@id": 8035, 
  "@type": "ScholarlyArticle", 
  "creator": [
    {
      "@type": "Person", 
      "affiliation": "Gebze Tech Univ, Dept Mol Biol & Genet, Kocaeli, Turkey", 
      "name": "Kayihan, Doga Selin"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Baskent Univ, Dept Mol Biol & Genet, Ankara, Turkey", 
      "name": "Kayihan, Ceyhun"
    }, 
    {
      "@type": "Person", 
      "affiliation": "Gebze Tech Univ, Dept Mol Biol & Genet, Kocaeli, Turkey", 
      "name": "Ciftci, Yelda Ozden"
    }
  ], 
  "datePublished": "2021-01-01", 
  "description": "Plants encounter many environmental factors such as low and high temperatures during phytoremediation processes. In this study, our aim was to produce the transgenic tobacco plants by using a newly characterized bacterial nitroreductase,Ntr, which was active at a broad range temperature in order to detoxify 2,4-dinitrotoluene (2,4-DNT) at lower temperature. The presence ofNtrand its heterologous expression was verified in T1 transgenic plants and their growing ability were determined under toxic amount of 2,4-DNT (35 mu M). Fresh weight and dry weight of transgenic plants were significantly higher than wild type (WT) under toxic 2,4-DNT at 22 degrees C, indicating higher growth capacity of the transgenics. Transgenic plants also showed a higher tolerance than WT when exposed to 2,4-DNT at 15 degrees C. Moreover, transformation rate of 2,4-DNT was gradually decreased through decreasing temperatures in WT media, however, it was increased through decreasing temperatures in transgenic plant TR3-25 media and it had the highest transformation rate (54%) of 2,4-DNT at 4 degrees C. Correlatively, 2,4-DNT treatment at 4 degrees C led to a significant decrease in H(2)O(2)level in transgenic plants. Thus, transgenic plants overexpressing nitroreductase might have an important advantage for phytoremediation of toxic nitroaromatic compounds in field applications at low temperatures.", 
  "headline": "Transgenic tobacco plants overexpressing a cold-adaptive nitroreductase gene exhibited enhanced 2,4-dinitrotoluene detoxification rate at low temperature", 
  "identifier": 8035, 
  "image": "https://aperta.ulakbim.gov.tr/static/img/logo/aperta_logo_with_icon.svg", 
  "license": "http://www.opendefinition.org/licenses/cc-by", 
  "name": "Transgenic tobacco plants overexpressing a cold-adaptive nitroreductase gene exhibited enhanced 2,4-dinitrotoluene detoxification rate at low temperature", 
  "url": "https://aperta.ulakbim.gov.tr/record/8035"
}