1 Ocak 2021 Dergi makalesi Açık Erişim

Kayihan, Doga Selin; Kayihan, Ceyhun; Ciftci, Yelda Ozden

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  <identifier identifierType="URL">https://aperta.ulakbim.gov.tr/record/8035</identifier>
  <creators>
    <creator>
      <creatorName>Kayihan, Doga Selin</creatorName>
      <givenName>Doga Selin</givenName>
      <familyName>Kayihan</familyName>
      <affiliation>Gebze Tech Univ, Dept Mol Biol &amp; Genet, Kocaeli, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Kayihan, Ceyhun</creatorName>
      <givenName>Ceyhun</givenName>
      <familyName>Kayihan</familyName>
      <affiliation>Baskent Univ, Dept Mol Biol &amp; Genet, Ankara, Turkey</affiliation>
    </creator>
    <creator>
      <creatorName>Ciftci, Yelda Ozden</creatorName>
      <givenName>Yelda Ozden</givenName>
      <familyName>Ciftci</familyName>
      <affiliation>Gebze Tech Univ, Dept Mol Biol &amp; Genet, Kocaeli, Turkey</affiliation>
    </creator>
  </creators>
  <titles>
    <title>Transgenic Tobacco Plants Overexpressing A Cold-Adaptive Nitroreductase Gene Exhibited Enhanced 2,4-Dinitrotoluene Detoxification Rate At Low Temperature</title>
  </titles>
  <publisher>Aperta</publisher>
  <publicationYear>2021</publicationYear>
  <dates>
    <date dateType="Issued">2021-01-01</date>
  </dates>
  <resourceType resourceTypeGeneral="Text">Journal article</resourceType>
  <alternateIdentifiers>
    <alternateIdentifier alternateIdentifierType="url">https://aperta.ulakbim.gov.tr/record/8035</alternateIdentifier>
  </alternateIdentifiers>
  <relatedIdentifiers>
    <relatedIdentifier relatedIdentifierType="DOI" relationType="IsIdenticalTo">10.1080/15226514.2020.1786795</relatedIdentifier>
  </relatedIdentifiers>
  <rightsList>
    <rights rightsURI="http://www.opendefinition.org/licenses/cc-by">Creative Commons Attribution</rights>
    <rights rightsURI="info:eu-repo/semantics/openAccess">Open Access</rights>
  </rightsList>
  <descriptions>
    <description descriptionType="Abstract">Plants encounter many environmental factors such as low and high temperatures during phytoremediation processes. In this study, our aim was to produce the transgenic tobacco plants by using a newly characterized bacterial nitroreductase,Ntr, which was active at a broad range temperature in order to detoxify 2,4-dinitrotoluene (2,4-DNT) at lower temperature. The presence ofNtrand its heterologous expression was verified in T1 transgenic plants and their growing ability were determined under toxic amount of 2,4-DNT (35 mu M). Fresh weight and dry weight of transgenic plants were significantly higher than wild type (WT) under toxic 2,4-DNT at 22 degrees C, indicating higher growth capacity of the transgenics. Transgenic plants also showed a higher tolerance than WT when exposed to 2,4-DNT at 15 degrees C. Moreover, transformation rate of 2,4-DNT was gradually decreased through decreasing temperatures in WT media, however, it was increased through decreasing temperatures in transgenic plant TR3-25 media and it had the highest transformation rate (54%) of 2,4-DNT at 4 degrees C. Correlatively, 2,4-DNT treatment at 4 degrees C led to a significant decrease in H(2)O(2)level in transgenic plants. Thus, transgenic plants overexpressing nitroreductase might have an important advantage for phytoremediation of toxic nitroaromatic compounds in field applications at low temperatures.</description>
  </descriptions>
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